Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 4025354..4025951 Replicon chromosome
Accession NZ_CP018735
Organism Klebsiella pneumoniae strain Kp_Goe_121641

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag BB745_RS20880 Protein ID WP_004142563.1
Coordinates 4025634..4025951 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag BB745_RS20875 Protein ID WP_004142561.1
Coordinates 4025354..4025641 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BB745_RS20845 4021435..4021683 + 249 WP_002893037.1 DUF1158 domain-containing protein -
BB745_RS20850 4021701..4022042 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
BB745_RS20855 4022073..4023188 - 1116 Protein_3885 MBL fold metallo-hydrolase -
BB745_RS20860 4023367..4023948 + 582 WP_032419262.1 TetR/AcrR family transcriptional regulator -
BB745_RS20865 4023948..4024316 + 369 WP_004142557.1 MmcQ/YjbR family DNA-binding protein -
BB745_RS20870 4024436..4025089 + 654 WP_032419263.1 oxygen-insensitive NAD(P)H nitroreductase -
BB745_RS20875 4025354..4025641 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
BB745_RS20880 4025634..4025951 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
BB745_RS20885 4026136..4027179 - 1044 WP_032419264.1 DUF2157 domain-containing protein -
BB745_RS20890 4027846..4028712 - 867 WP_032419265.1 helix-turn-helix transcriptional regulator -
BB745_RS20895 4028821..4030248 + 1428 WP_004176980.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T70382 WP_004142563.1 NZ_CP018735:c4025951-4025634 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T70382 NZ_CP018735:c4025951-4025634 [Klebsiella pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT70382 WP_004142561.1 NZ_CP018735:c4025641-4025354 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT70382 NZ_CP018735:c4025641-4025354 [Klebsiella pneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCGACAGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References