Detailed information of TA system TA070366

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 3340850..3341365
• Replicon: chromosome
• Accession: NZ_CP018728
• Organism: Xanthomonas hortorum pv. gardneri strain JS749-3

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A1L5Y3A2
• Locus tag: BJD10_RS15170
• Protein ID: WP_006450902.1
• Coordinates: 3341159..3341365 (-)
• Length: 69 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: BJD10_RS15165
• Protein ID: WP_006450903.1
• Coordinates: 3340850..3341149 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BJD10_RS15140	3336296..3337180	+	885	WP_006451802.1	MinD/ParA family protein	-
BJD10_RS15145	3337177..3337950	+	774	WP_023903918.1	RNA polymerase sigma factor FliA	-
BJD10_RS15150	3337986..3338378	+	393	WP_002813536.1	chemotaxis response regulator CheY	-
BJD10_RS15155	3338378..3339004	+	627	WP_006450905.1	protein phosphatase CheZ	-
BJD10_RS15160	3339007..3340665	+	1659	WP_006450904.1	chemotaxis protein CheA	-
BJD10_RS15165	3340850..3341149	-	300	WP_006450903.1	HigA family addiction module antidote protein	Antitoxin
BJD10_RS15170	3341159..3341365	-	207	WP_006450902.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BJD10_RS15175	3342278..3343018	+	741	WP_006452732.1	flagellar motor protein	-
BJD10_RS15180	3343026..3344015	+	990	WP_006452731.1	flagellar motor protein MotD	-
BJD10_RS15185	3344017..3344799	+	783	WP_006452730.1	ParA family protein	-
BJD10_RS15190	3344796..3346046	+	1251	WP_046933744.1	chemotaxis protein CheW	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	cheY	3333986..3352753	18767

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (3-68)

Antitoxin

• Cro/C1-type helix-turn-helix domain (13-65)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7818.83 Da
• Isoelectric Point: 6.2041

>T70366 WP_006450902.1 NZ_CP018728:c3341365-3341159 [Xanthomonas hortorum pv. gardneri]
MQAVAERKLQLLDSAQTLEFLRSPPGNRFELLAGTRAGQHSIRINDQWRVCFVWTDAGPEHVEIVDYH

Nucleotide

• Length: 207 bp

>T70366 NZ_CP018728:c3341365-3341159 [Xanthomonas hortorum pv. gardneri]
ATGCAAGCTGTAGCCGAACGGAAGCTGCAATTGCTGGATTCGGCGCAGACGCTGGAGTTCTTGCGCAGCCCGCCGGGCAA
TCGGTTTGAGCTTCTTGCGGGTACGCGTGCAGGTCAGCACAGCATCCGCATCAATGACCAATGGCGTGTGTGCTTTGTCT
GGACTGACGCCGGCCCCGAGCACGTTGAGATTGTCGATTACCACTGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10774.38 Da
• Isoelectric Point: 10.1958

>AT70366 WP_006450903.1 NZ_CP018728:c3341149-3340850 [Xanthomonas hortorum pv. gardneri]
MRPNNKLRAIHPGEILREDFMLPLGLTINGLARALDVPATRIHSIVHGERAISADTAARLARHFGGDAASWLALQATYDL
KTLPSTGEIERNVQPRVAA

Nucleotide

• Length: 300 bp

>AT70366 NZ_CP018728:c3341149-3340850 [Xanthomonas hortorum pv. gardneri]
ATGCGACCCAACAACAAACTGCGCGCCATCCATCCCGGCGAAATCCTGCGGGAAGATTTCATGCTGCCGCTGGGGCTGAC
CATCAACGGCCTGGCGCGGGCGCTGGATGTACCGGCAACGCGCATCCACAGCATCGTGCATGGCGAGCGTGCCATCAGCG
CCGATACCGCAGCGCGGCTTGCACGCCACTTCGGTGGCGATGCGGCGTCCTGGCTCGCGCTACAGGCCACCTACGACCTG
AAGACCTTGCCTAGCACCGGCGAGATCGAGCGCAACGTGCAGCCGCGTGTGGCGGCCTAG

Structures

Toxin

• AlphaFold DB: A0A1L5Y3A2