Detailed information of TA system TA070363

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 641958..642547
• Replicon: chromosome
• Accession: NZ_CP018728
• Organism: Xanthomonas hortorum pv. gardneri strain JS749-3

Toxin (Protein)

• Gene name: graT
• Uniprot ID: F0C464
• Locus tag: BJD10_RS02840
• Protein ID: WP_006449988.1
• Coordinates: 641958..642239 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: BJD10_RS02845
• Protein ID: WP_006449989.1
• Coordinates: 642257..642547 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BJD10_RS02830	638328..640550	+	2223	Protein_533	exodeoxyribonuclease V subunit alpha	-
BJD10_RS02835	640689..641744	+	1056	WP_006449987.1	virulence RhuM family protein	-
BJD10_RS02840	641958..642239	+	282	WP_006449988.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BJD10_RS02845	642257..642547	+	291	WP_006449989.1	HigA family addiction module antidote protein	Antitoxin
BJD10_RS02850	643081..643413	-	333	WP_006449991.1	hypothetical protein	-
BJD10_RS02855	644055..645650	+	1596	WP_006449992.1	deoxycytidylate deaminase	-
BJD10_RS02860	645866..646846	-	981	WP_074065938.1	IS5 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	645866..646846	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-93)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (6-82)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11044.69 Da
• Isoelectric Point: 9.7972

>T70363 WP_006449988.1 NZ_CP018728:641958-642239 [Xanthomonas hortorum pv. gardneri]
VIRSFIDKEAEKIWLGERSRRLPSDIQSVARRKLRMLNAAAHLDDLRIPPANRLEALKGERRGQYSIRINDQWRICFRWM
EGDVAEVEIVDYH

Nucleotide

• Length: 282 bp

>T70363 NZ_CP018728:641958-642239 [Xanthomonas hortorum pv. gardneri]
GTGATCAGGAGCTTTATCGACAAGGAGGCCGAAAAGATCTGGCTGGGTGAGCGCTCCCGCCGGTTGCCGTCCGACATCCA
GTCGGTCGCGCGCCGCAAGTTGCGCATGCTCAACGCTGCCGCGCACCTCGACGATCTGCGCATTCCGCCCGCCAACCGGC
TGGAAGCGTTGAAGGGCGAGCGGCGCGGGCAGTACAGCATCCGGATCAACGACCAATGGCGCATCTGCTTCCGATGGATG
GAGGGCGATGTCGCCGAGGTCGAAATCGTCGATTACCACTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10540.20 Da
• Isoelectric Point: 6.4664

>AT70363 WP_006449989.1 NZ_CP018728:642257-642547 [Xanthomonas hortorum pv. gardneri]
MTVLPNIHPGEILLEEFLEPMGISQNALARATDVPPRRINEIVLGKRGITADTAVRLAAALGTTERFWLGLQADYELEQA
HRALGDLPSRIKRLAA

Nucleotide

• Length: 291 bp

>AT70363 NZ_CP018728:642257-642547 [Xanthomonas hortorum pv. gardneri]
ATGACAGTCCTACCCAACATCCACCCCGGCGAAATTCTGCTCGAAGAGTTTCTCGAGCCGATGGGCATCAGCCAGAACGC
ACTGGCGCGGGCCACCGATGTACCGCCGCGCCGCATCAACGAGATCGTGCTGGGCAAGCGCGGCATCACTGCCGACACCG
CCGTACGCCTGGCTGCCGCGTTGGGCACGACCGAGCGCTTCTGGTTGGGCCTGCAGGCCGATTACGAGCTGGAACAAGCG
CATCGCGCGCTGGGCGATCTGCCGTCACGGATCAAGCGGTTGGCGGCTTGA

Structures

Toxin

• AlphaFold DB: A0A6V7F8V8