Detailed information of TA system TA070337

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 37271..37928
• Replicon: plasmid pKp_Goe_021-2
• Accession: NZ_CP018716
• Organism: Klebsiella pneumoniae strain Kp_Goe_152021

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U3PDC3
• Locus tag: BB786_RS29635
• Protein ID: WP_000270043.1
• Coordinates: 37578..37928 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: BB786_RS29630
• Protein ID: WP_000124640.1
• Coordinates: 37271..37573 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BB786_RS29580	32896..33324	+	429	WP_000591074.1	hypothetical protein	-
BB786_RS29585	33381..33740	+	360	WP_000422768.1	hypothetical protein	-
BB786_RS29590	33740..34186	+	447	WP_000919345.1	Fe3+-siderophore ABC transporter permease	-
BB786_RS29595	34183..34701	+	519	WP_000210756.1	nitrite reductase	-
BB786_RS29600	34701..34931	+	231	WP_000972663.1	hypothetical protein	-
BB786_RS29605	34918..35775	+	858	WP_001167032.1	hypothetical protein	-
BB786_RS29615	36006..36533	+	528	WP_001236377.1	thermonuclease family protein	-
BB786_RS29620	36591..36863	+	273	WP_001043047.1	HU family DNA-binding protein	-
BB786_RS29625	36951..37244	+	294	WP_001239997.1	chromosome segregation protein ParM	-
BB786_RS29630	37271..37573	-	303	WP_000124640.1	XRE family transcriptional regulator	Antitoxin
BB786_RS29635	37578..37928	-	351	WP_000270043.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BB786_RS29640	38091..38639	+	549	WP_001061574.1	transcriptional regulator	-
BB786_RS29650	38980..39174	+	195	WP_000343597.1	hypothetical protein	-
BB786_RS29655	39185..39556	+	372	WP_000516916.1	hypothetical protein	-
BB786_RS29660	39549..40019	+	471	WP_001281821.1	hypothetical protein	-
BB786_RS29665	40034..40369	-	336	WP_000683476.1	hypothetical protein	-
BB786_RS29670	40466..40954	+	489	WP_001273096.1	DUF1643 domain-containing protein	-
BB786_RS29675	40957..41454	+	498	WP_000062185.1	hypothetical protein	-
BB786_RS29685	41669..42373	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
BB786_RS29690	42438..42617	-	180	Protein_53	helix-turn-helix transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aac(6')-Ib / qacE / sul1 / floR / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2	-	1..96074	96074

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13328.21 Da
• Isoelectric Point: 5.2421

>T70337 WP_000270043.1 NZ_CP018716:c37928-37578 [Klebsiella pneumoniae]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Nucleotide

• Length: 351 bp

>T70337 NZ_CP018716:c37928-37578 [Klebsiella pneumoniae]
ATGTGGGTCATCGAGACAACCGACACCTTTGATGAGTGGTTCGATGCTCTGGATGATACCGATAGAGCAAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGCCCCATGCTGTCGAGGCCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTTCGGGTCCAAAGCAAAGGAGATCCTATCAGAGCGTTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGTGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11006.73 Da
• Isoelectric Point: 7.2036

>AT70337 WP_000124640.1 NZ_CP018716:c37573-37271 [Klebsiella pneumoniae]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Nucleotide

• Length: 303 bp

>AT70337 NZ_CP018716:c37573-37271 [Klebsiella pneumoniae]
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA

Structures

Toxin

• AlphaFold DB: A0A7G8AFB9