Detailed information of TA system TA070227

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 65561..66218
• Replicon: plasmid pKp_Goe_473-2
• Accession: NZ_CP018689
• Organism: Klebsiella pneumoniae strain Kp_Goe_149473

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U3PDC3
• Locus tag: BB750_RS29865
• Protein ID: WP_000270043.1
• Coordinates: 65561..65911 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: BB750_RS29870
• Protein ID: WP_000124640.1
• Coordinates: 65916..66218 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BB750_RS29810	60872..61051	+	180	Protein_80	helix-turn-helix transcriptional regulator	-
BB750_RS29815	61116..61820	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
BB750_RS29825	62035..62532	-	498	WP_000062185.1	hypothetical protein	-
BB750_RS29830	62535..63023	-	489	WP_001273096.1	DUF1643 domain-containing protein	-
BB750_RS29835	63120..63455	+	336	WP_000683476.1	hypothetical protein	-
BB750_RS29840	63470..63940	-	471	WP_001281821.1	hypothetical protein	-
BB750_RS29845	63933..64304	-	372	WP_000516916.1	hypothetical protein	-
BB750_RS29850	64315..64509	-	195	WP_000343597.1	hypothetical protein	-
BB750_RS29860	64850..65398	-	549	WP_001061574.1	transcriptional regulator	-
BB750_RS29865	65561..65911	+	351	WP_000270043.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BB750_RS29870	65916..66218	+	303	WP_000124640.1	XRE family transcriptional regulator	Antitoxin
BB750_RS29875	66245..66538	-	294	WP_001239997.1	chromosome segregation protein ParM	-
BB750_RS29880	66626..66898	-	273	WP_001043047.1	HU family DNA-binding protein	-
BB750_RS29885	66956..67483	-	528	WP_001236377.1	thermonuclease family protein	-
BB750_RS29895	67714..68571	-	858	WP_001167032.1	hypothetical protein	-
BB750_RS29900	68558..68788	-	231	WP_000972663.1	hypothetical protein	-
BB750_RS29905	68788..69306	-	519	WP_000210756.1	nitrite reductase	-
BB750_RS29910	69303..69749	-	447	WP_000919345.1	Fe3+-siderophore ABC transporter permease	-
BB750_RS29915	69749..70108	-	360	WP_000422768.1	hypothetical protein	-
BB750_RS29920	70165..70593	-	429	WP_000591074.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sul2 / aph(3'')-Ib / aph(6)-Id / tet(A) / floR / sul1 / qacE / aac(6')-Ib	-	1..96070	96070

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13328.21 Da
• Isoelectric Point: 5.2421

>T70227 WP_000270043.1 NZ_CP018689:65561-65911 [Klebsiella pneumoniae]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Nucleotide

• Length: 351 bp

>T70227 NZ_CP018689:65561-65911 [Klebsiella pneumoniae]
ATGTGGGTCATCGAGACAACCGACACCTTTGATGAGTGGTTCGATGCTCTGGATGATACCGATAGAGCAAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGCCCCATGCTGTCGAGGCCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTTCGGGTCCAAAGCAAAGGAGATCCTATCAGAGCGTTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGTGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11006.73 Da
• Isoelectric Point: 7.2036

>AT70227 WP_000124640.1 NZ_CP018689:65916-66218 [Klebsiella pneumoniae]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Nucleotide

• Length: 303 bp

>AT70227 NZ_CP018689:65916-66218 [Klebsiella pneumoniae]
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA

Structures

Toxin

• AlphaFold DB: A0A7G8AFB9