Detailed information of TA system TA070170

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 2516697..2517343
• Replicon: chromosome
• Accession: NZ_CP018671
• Organism: Klebsiella pneumoniae strain CAV1042

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A483JFR4
• Locus tag: AGE78_RS15255
• Protein ID: WP_004188313.1
• Coordinates: 2516996..2517343 (-)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AGE78_RS15250
• Protein ID: WP_064179144.1
• Coordinates: 2516697..2516996 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AGE78_RS15230	2512903..2513661	-	759	WP_002920548.1	DeoR/GlpR family transcriptional regulator	-
AGE78_RS15235	2513711..2514541	-	831	WP_071557142.1	rhomboid family intramembrane serine protease GlpG	-
AGE78_RS15240	2514592..2514921	-	330	WP_002920552.1	thiosulfate sulfurtransferase GlpE	-
AGE78_RS15245	2515126..2516634	+	1509	WP_071557143.1	glycerol-3-phosphate dehydrogenase	-
AGE78_RS15250	2516697..2516996	-	300	WP_064179144.1	helix-turn-helix domain-containing protein	Antitoxin
AGE78_RS15255	2516996..2517343	-	348	WP_004188313.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AGE78_RS15260	2517509..2519956	-	2448	WP_071557144.1	glycogen phosphorylase	-
AGE78_RS15265	2519974..2521407	-	1434	WP_002920564.1	glycogen synthase GlgA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-107)

Antitoxin

• Cro/C1-type helix-turn-helix domain (31-84)

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 13548.57 Da
• Isoelectric Point: 6.2327

>T70170 WP_004188313.1 NZ_CP018671:c2517343-2516996 [Klebsiella pneumoniae]
MWDVETTDTFDTWFELQSRALKEDMLATMLILSEFAPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRAFFAFDPLRKAI
VLCAGNKDGMNEKRFYKEMITLADREFSQHLTKER

Nucleotide

• Length: 348 bp

>T70170 NZ_CP018671:c2517343-2516996 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATACCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTCCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTTTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGCGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10887.67 Da
• Isoelectric Point: 10.4022

>AT70170 WP_064179144.1 NZ_CP018671:c2516996-2516697 [Klebsiella pneumoniae]
MATLKELMAKQSPESQQRIAAKAAERRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQTDNDPRLSTLKRYIAALG
GELSINVTLPTGKKVSFNL

Nucleotide

• Length: 300 bp

>AT70170 NZ_CP018671:c2516996-2516697 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGAAAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGAGACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGACTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Structures

Toxin

• AlphaFold DB: A0A483JFR4