Detailed information of TA system TA070118

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): holin-SprF3/-
• Location: 2129345..2129793
• Replicon: chromosome
• Accession: NZ_CP018629
• Organism: Staphylococcus aureus strain MRSA107

Toxin (Protein)

• Gene name: holin
• Uniprot ID: A0A853P973
• Locus tag: BTN44_RS11310
• Protein ID: WP_000387656.1
• Coordinates: 2129345..2129647 (-)
• Length: 101 a.a.

Antitoxin (RNA)

Gene name	SprF3
Locus tag	-
Coordinates	2129727..2129793 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BTN44_RS11280	2124452..2124550	-	99	WP_001803958.1	hypothetical protein	-
BTN44_RS11285	2124646..2125860	+	1215	WP_000286469.1	NADH-dependent flavin oxidoreductase	-
BTN44_RS11290	2125918..2126745	+	828	WP_000136023.1	alpha/beta hydrolase	-
BTN44_RS11305	2127879..2129333	-	1455	WP_103067904.1	N-acetylmuramoyl-L-alanine amidase	-
BTN44_RS11310	2129345..2129647	-	303	WP_000387656.1	phage holin	Toxin
BTN44_RS11315	2129698..2129802	+	105	Protein_2093	hypothetical protein	-
-	2129727..2129793	+	67	NuclAT_1	-	Antitoxin
-	2129727..2129793	+	67	NuclAT_1	-	Antitoxin
-	2129727..2129793	+	67	NuclAT_1	-	Antitoxin
-	2129727..2129793	+	67	NuclAT_1	-	Antitoxin
BTN44_RS16600	2129846..2129993	-	148	Protein_2094	putative holin-like toxin	-
BTN44_RS11325	2130185..2130481	-	297	WP_000539688.1	DUF2951 domain-containing protein	-
BTN44_RS11330	2130539..2130826	-	288	WP_001040261.1	hypothetical protein	-
BTN44_RS11335	2130873..2131025	-	153	WP_001153681.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	hlb / groEL	2127879..2185469	57590

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Holin (3-68)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11177.94 Da
• Isoelectric Point: 9.6587

>T70118 WP_000387656.1 NZ_CP018629:c2129647-2129345 [Staphylococcus aureus]
MEAKVMARYVVLILALVNQFLANKGISPIPVDEESISSIILTVIALYTTYKDNPTSQEGRWANQKLKKYKAESKYRKATG
QAPIKEVMTPTNMNDTNDLG

Nucleotide

• Length: 303 bp

>T70118 NZ_CP018629:c2129647-2129345 [Staphylococcus aureus]
ATGGAAGCAAAAGTAATGGCAAGATACGTTGTATTGATTTTAGCGTTAGTCAATCAGTTTTTAGCGAATAAAGGTATAAG
CCCGATACCAGTAGATGAAGAAAGTATTTCATCGATCATCTTAACAGTTATTGCTTTATACACTACGTATAAAGACAATC
CAACATCACAAGAAGGACGTTGGGCGAATCAAAAATTAAAGAAATATAAAGCAGAAAGTAAATATAGAAAAGCAACAGGA
CAAGCACCTATCAAAGAAGTGATGACACCTACAAATATGAACGACACAAATGATTTAGGGTAG

Antitoxin

Nucleotide

• Length: 67 bp

>AT70118 NZ_CP018629:2129727-2129793 [Staphylococcus aureus]
ATAAATAGAAAAAGGGCAACATGCGGAAACATGTTACCCTAGTGAGCCCGTTAAAAAGACGGTGGCT

Structures

Toxin

• AlphaFold DB: A0A853P973

Antitoxin