Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type I Classification (family/domain) hok-sok/-
Location 79252..79516 Replicon plasmid pFORC44_1
Accession NZ_CP018625
Organism Escherichia coli strain FORC_044

Toxin (Protein)


Gene name pndA Uniprot ID U9Y6M3
Locus tag FORC44_RS29930 Protein ID WP_001331364.1
Coordinates 79364..79516 (+) Length 51 a.a.

Antitoxin (RNA)


Gene name pndB
Locus tag -
Coordinates 79252..79309 (-)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FORC44_RS29915 74491..76782 - 2292 WP_001289276.1 hypothetical protein -
FORC44_RS29920 76775..77845 - 1071 WP_000151583.1 IncI1-type conjugal transfer protein TrbB -
FORC44_RS29925 77864..79072 - 1209 WP_000121274.1 IncI1-type conjugal transfer protein TrbA -
- 79252..79309 - 58 NuclAT_0 - Antitoxin
- 79252..79309 - 58 NuclAT_0 - Antitoxin
- 79252..79309 - 58 NuclAT_0 - Antitoxin
- 79252..79309 - 58 NuclAT_0 - Antitoxin
FORC44_RS29930 79364..79516 + 153 WP_001331364.1 Hok/Gef family protein Toxin
FORC44_RS31695 79588..79728 - 141 WP_000880644.1 hypothetical protein -
FORC44_RS29935 80139..80435 + 297 WP_001275298.1 hypothetical protein -
FORC44_RS31700 80500..80676 - 177 WP_001054904.1 hypothetical protein -
FORC44_RS29940 81068..81277 + 210 WP_000062603.1 HEAT repeat domain-containing protein -
FORC44_RS29945 81349..82011 - 663 WP_000653334.1 plasmid IncI1-type surface exclusion protein ExcA -
FORC44_RS29950 82076..84238 - 2163 WP_000698351.1 DotA/TraY family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aph(3')-Ia / aph(6)-Id / aph(3'')-Ib / sul2 / blaTEM-1B - 1..111755 111755


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Hok/gef cell toxic protein

(7-46)


Sequences

Toxin        


Download         Length: 51 a.a.        Molecular weight: 5775.13 Da        Isoelectric Point: 8.7948

>T70103 WP_001331364.1 NZ_CP018625:79364-79516 [Escherichia coli]
MPQRTFLMMLIVICVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Download         Length: 153 bp

>T70103 NZ_CP018625:79364-79516 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCATCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGGACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin


Download         Length: 58 bp

>AT70103 NZ_CP018625:c79309-79252 [Escherichia coli]
GCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5W9M382

Antitoxin

Download structure file

References