Detailed information of TA system TA070069

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 136377..136803
• Replicon: plasmid unnamed
• Accession: NZ_CP018455
• Organism: Klebsiella pneumoniae strain SWU01

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: BSL40_RS30200
• Protein ID: WP_001312861.1
• Coordinates: 136377..136535 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	136579..136803 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BSL40_RS30155	131432..131659	-	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-
BSL40_RS30160	131753..132439	-	687	WP_015059009.1	PAS domain-containing protein	-
BSL40_RS30165	132630..133013	-	384	WP_000124981.1	relaxosome protein TraM	-
BSL40_RS30170	133290..133937	+	648	WP_015059008.1	transglycosylase SLT domain-containing protein	-
BSL40_RS30175	134234..135055	-	822	WP_001234445.1	DUF945 domain-containing protein	-
BSL40_RS30180	135166..135462	-	297	WP_001272251.1	hypothetical protein	-
BSL40_RS32065	135762..136139	+	378	Protein_176	hypothetical protein	-
BSL40_RS30200	136377..136535	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	136579..136803	-	225	NuclAT_0	-	Antitoxin
-	136579..136803	-	225	NuclAT_0	-	Antitoxin
-	136579..136803	-	225	NuclAT_0	-	Antitoxin
-	136579..136803	-	225	NuclAT_0	-	Antitoxin
BSL40_RS30205	136815..137534	-	720	WP_001276217.1	plasmid SOS inhibition protein A	-
BSL40_RS30210	137531..137965	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
BSL40_RS30215	138034..140057	-	2024	Protein_180	ParB/RepB/Spo0J family partition protein	-
BSL40_RS30220	140118..140351	-	234	WP_000006003.1	DUF905 family protein	-
BSL40_RS30225	140409..140936	-	528	WP_000290834.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-65 / blaSHV-12 / blaKPC-2 / rmtB / blaTEM-1B	-	1..162552	162552

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T70069 WP_001312861.1 NZ_CP018455:c136535-136377 [Klebsiella pneumoniae]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T70069 NZ_CP018455:c136535-136377 [Klebsiella pneumoniae]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT70069 NZ_CP018455:c136803-136579 [Klebsiella pneumoniae]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin