Detailed information of TA system TA070065

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 99820..100073
• Replicon: plasmid unnamed
• Accession: NZ_CP018455
• Organism: Klebsiella pneumoniae strain SWU01

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: BSL40_RS29945
• Protein ID: WP_001312851.1
• Coordinates: 99924..100073 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	99820..99879 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BSL40_RS29905	95480..95545	-	66	Protein_123	helix-turn-helix domain-containing protein	-
BSL40_RS29915	95598..96302	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
BSL40_RS30835	96366..96527	+	162	Protein_125	DNA helicase	-
BSL40_RS29920	96547..97293	+	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
BSL40_RS29925	97348..97908	+	561	WP_000139328.1	fertility inhibition protein FinO	-
BSL40_RS29930	98040..98240	+	201	WP_015059022.1	hypothetical protein	-
BSL40_RS29935	98626..99225	+	600	WP_032083981.1	hypothetical protein	-
BSL40_RS29940	99389..99619	+	231	WP_001736714.1	hypothetical protein	-
-	99820..99879	-	60	NuclAT_1	-	Antitoxin
-	99820..99879	-	60	NuclAT_1	-	Antitoxin
-	99820..99879	-	60	NuclAT_1	-	Antitoxin
-	99820..99879	-	60	NuclAT_1	-	Antitoxin
BSL40_RS29945	99924..100073	+	150	WP_001312851.1	type I toxin-antitoxin system Hok family toxin	Toxin
BSL40_RS29950	100357..100605	+	249	WP_000083837.1	replication regulatory protein RepA	-
BSL40_RS29960	100850..100924	+	75	WP_001375168.1	RepA leader peptide Tap	-
BSL40_RS29965	100917..101774	+	858	WP_032495102.1	incFII family plasmid replication initiator RepA	-
BSL40_RS32060	102713..102877	+	165	WP_187417362.1	hypothetical protein	-
BSL40_RS29985	102914..103618	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-65 / blaSHV-12 / blaKPC-2 / rmtB / blaTEM-1B	-	1..162552	162552
	-	flank	IS/Tn	-	-	102914..103618	704

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T70065 WP_001312851.1 NZ_CP018455:99924-100073 [Klebsiella pneumoniae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T70065 NZ_CP018455:99924-100073 [Klebsiella pneumoniae]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGTGCCACGGTATTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 60 bp

>AT70065 NZ_CP018455:c99879-99820 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin