Detailed information of TA system TA069848

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 4725663..4726372
• Replicon: chromosome
• Accession: NZ_CP018363
• Organism: Mycobacterium avium subsp. hominissuis strain H87

Toxin (Protein)

• Gene name: HigB2
• Uniprot ID: X7V9P5
• Locus tag: BS641_RS22140
• Protein ID: WP_003873387.1
• Coordinates: 4725663..4726067 (+)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: HigA2
• Uniprot ID: X7V856
• Locus tag: BS641_RS22145
• Protein ID: WP_019732445.1
• Coordinates: 4726064..4726372 (+)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BS641_RS22115	4720994..4721572	-	579	WP_011726026.1	sigma-70 family RNA polymerase sigma factor	-
BS641_RS22120	4721663..4722475	-	813	WP_170945464.1	hypothetical protein	-
BS641_RS22125	4722874..4723182	+	309	WP_003874400.1	WhiB family transcriptional regulator	-
BS641_RS22130	4723225..4723989	+	765	WP_023900746.1	superoxide dismutase family protein	-
BS641_RS22135	4724146..4725345	-	1200	WP_075362332.1	FAD-dependent oxidoreductase	-
BS641_RS22140	4725663..4726067	+	405	WP_003873387.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BS641_RS22145	4726064..4726372	+	309	WP_019732445.1	helix-turn-helix transcriptional regulator	Antitoxin
BS641_RS22155	4726859..4728385	-	1527	WP_019732444.1	adenylate/guanylate cyclase domain-containing protein	-
BS641_RS22160	4728480..4730096	-	1617	WP_003873390.1	chaperonin GroEL	-
BS641_RS22165	4730185..4730487	-	303	WP_019732443.1	co-chaperone GroES	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-109)

Antitoxin

• HigA2-like, helix-turn-helix domain (32-91)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 15503.82 Da
• Isoelectric Point: 10.3815

>T69848 WP_003873387.1 NZ_CP018363:4725663-4726067 [Mycobacterium avium subsp. hominissuis]
VRKWEVDLTLIEAWMDALDDEEYDNLIAALEQLEEHGPITRRPFVDTLEGSRHPNMKELRPRPTKAGAHIRVLFAFDTRS
RAIMLIAGDKAGNWSKWYAKHIPIADELFDAHQKRLHKAAAKATNRKPRKGKKR

Nucleotide

• Length: 405 bp

>T69848 NZ_CP018363:4725663-4726067 [Mycobacterium avium subsp. hominissuis]
GTGCGGAAATGGGAGGTTGACCTGACTCTCATCGAAGCCTGGATGGACGCCCTCGATGACGAGGAGTACGACAACTTGAT
AGCAGCGCTCGAGCAGTTGGAGGAACACGGTCCAATTACCCGGCGGCCGTTTGTGGACACCCTTGAAGGGTCACGGCACC
CGAACATGAAGGAGCTCCGTCCGCGCCCCACGAAAGCTGGAGCCCACATTCGCGTGCTATTCGCCTTCGATACCCGGTCG
CGGGCGATCATGTTGATCGCTGGAGACAAGGCGGGGAATTGGTCCAAGTGGTATGCCAAGCACATTCCCATTGCCGACGA
GCTCTTCGATGCTCACCAAAAGCGTTTGCACAAGGCAGCAGCCAAAGCCACAAATCGGAAACCAAGGAAGGGTAAGAAAC
GATGA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11454.95 Da
• Isoelectric Point: 10.0802

>AT69848 WP_019732445.1 NZ_CP018363:4726064-4726372 [Mycobacterium avium subsp. hominissuis]
MTNLDDMRRRRPGNRTRIDAIKAEMDREVAHYRLRELREAAGYTQTTLAAAIGVGQNRVSQMEHGDLGTSRVDTLRKYVE
ATGGELEVSVKRPDGSRVLLSL

Nucleotide

• Length: 309 bp

>AT69848 NZ_CP018363:4726064-4726372 [Mycobacterium avium subsp. hominissuis]
ATGACCAATCTTGATGACATGCGCCGTCGCCGTCCCGGCAACCGGACCCGTATCGACGCGATCAAGGCGGAGATGGACCG
CGAGGTTGCCCACTACCGTCTCCGTGAACTGCGCGAGGCTGCCGGCTACACGCAGACGACTCTCGCTGCGGCTATTGGCG
TCGGGCAGAACCGCGTCTCGCAGATGGAACATGGCGATCTCGGCACCAGCCGGGTCGACACTCTCCGCAAGTACGTGGAA
GCGACTGGGGGTGAGCTGGAAGTGTCCGTCAAGCGTCCGGACGGGTCACGGGTTCTCCTCAGTCTGTAA

Structures

Toxin

• AlphaFold DB: X7V9P5

Antitoxin

• AlphaFold DB: X7V856