Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 236325..237049 Replicon plasmid pCAV1752-278
Accession NZ_CP018361
Organism Klebsiella oxytoca strain CAV1752

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag AGH21_RS02080 Protein ID WP_076752146.1
Coordinates 236738..237049 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag AGH21_RS02075 Protein ID WP_076752145.1
Coordinates 236325..236741 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AGH21_RS02030 231844..232320 + 477 WP_076752132.1 transglycosylase SLT domain-containing protein -
AGH21_RS02035 232358..232888 - 531 WP_076752134.1 antirestriction protein -
AGH21_RS02040 233080..233310 + 231 WP_076752135.1 hypothetical protein -
AGH21_RS02050 233514..234347 - 834 WP_076752137.1 N-6 DNA methylase -
AGH21_RS02055 234394..234624 - 231 WP_076752138.1 hypothetical protein -
AGH21_RS02060 234713..235060 - 348 WP_076752140.1 hypothetical protein -
AGH21_RS02065 235163..235435 - 273 WP_137055850.1 hypothetical protein -
AGH21_RS02075 236325..236741 - 417 WP_076752145.1 helix-turn-helix domain-containing protein Antitoxin
AGH21_RS02080 236738..237049 - 312 WP_076752146.1 type II toxin-antitoxin system HigB family toxin Toxin
AGH21_RS02085 237199..237411 - 213 WP_076752148.1 hypothetical protein -
AGH21_RS02090 237519..237845 - 327 WP_076752150.1 hypothetical protein -
AGH21_RS02095 238704..238862 - 159 WP_076752152.1 type I toxin-antitoxin system Hok family toxin -
AGH21_RS02100 238934..240007 + 1074 WP_013609528.1 IS481-like element ISKpn28 family transposase -
AGH21_RS02105 240305..240790 - 486 WP_076752153.1 GNAT family N-acetyltransferase -
AGH21_RS02110 240778..241044 - 267 WP_012540086.1 DUF1778 domain-containing protein -
AGH21_RS02115 241345..241668 + 324 WP_013087171.1 metalloregulator ArsR/SmtB family transcription factor -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..277616 277616
- flank IS/Tn - - 238934..240007 1073


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(21-93)

Antitoxin

Antitoxin HigA

(5-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12510.31 Da        Isoelectric Point: 9.9242

>T69825 WP_076752146.1 NZ_CP018361:c237049-236738 [Klebsiella oxytoca]
VHVISRAPFDEAARHYPNDAAAIDDTYRVLKRVVAKKPDELKRYFNSLDRMKYREKWWVIDIGGNNLRMMFFADFERGKI
FVKHITTHAEYDRLTKYYREHKE

Download         Length: 312 bp

>T69825 NZ_CP018361:c237049-236738 [Klebsiella oxytoca]
GTGCATGTTATATCACGAGCCCCTTTTGATGAGGCGGCGAGACACTATCCGAATGATGCAGCGGCCATCGATGACACATA
TCGTGTACTAAAGCGGGTTGTAGCAAAAAAACCGGATGAGCTAAAGCGATACTTTAATAGTCTGGATCGCATGAAATACC
GGGAAAAATGGTGGGTTATCGATATCGGCGGCAATAACCTCAGAATGATGTTTTTTGCTGATTTCGAGCGCGGAAAGATA
TTCGTCAAACACATTACCACCCATGCTGAATATGATCGCTTAACGAAATACTATCGGGAGCACAAAGAATGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15484.61 Da        Isoelectric Point: 4.4711

>AT69825 WP_076752145.1 NZ_CP018361:c236741-236325 [Klebsiella oxytoca]
MIFSDAIKAANDLASIVPLLGGSSSRKDYEEALKLVEYLLEHDPDSPLVDMLTARIDAWEDNAVEFEEFNTRIEAGKNGV
SLLRVLMQQHGLSQSDFENEIGKKSLVSRILSCERSLTLDHMRALANRFQIPVSMFVD

Download         Length: 417 bp

>AT69825 NZ_CP018361:c236741-236325 [Klebsiella oxytoca]
ATGATTTTTAGCGATGCCATTAAAGCTGCAAATGACCTTGCCAGTATTGTACCCCTGCTGGGTGGCAGCTCGTCCCGCAA
AGATTACGAAGAGGCACTTAAACTCGTTGAATATCTTCTGGAACATGATCCGGACAGCCCGCTGGTTGATATGCTGACTG
CCCGCATCGATGCCTGGGAAGACAACGCGGTGGAATTTGAAGAATTTAATACGCGCATCGAAGCAGGCAAAAACGGTGTC
TCATTGCTGCGGGTTCTTATGCAGCAACATGGGCTTTCACAGTCCGATTTTGAAAACGAGATAGGCAAAAAATCGCTCGT
CAGCCGGATCCTCAGTTGTGAACGCAGTCTGACCCTTGATCACATGCGAGCGCTGGCAAATCGATTTCAAATCCCGGTCT
CGATGTTTGTTGACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References