Detailed information of TA system TA069774

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 19898..20167
• Replicon: plasmid pKp_Goe_414-6
• Accession: NZ_CP018343
• Organism: Klebsiella pneumoniae isolate Kp_Goe_154414

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: BB788_RS30815
• Protein ID: WP_001312861.1
• Coordinates: 20009..20167 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	19898..19963 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BB788_RS30790	15608..16135	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
BB788_RS30795	16193..16426	+	234	WP_000006003.1	DUF905 family protein	-
BB788_RS30800	16487..18510	+	2024	Protein_22	ParB/RepB/Spo0J family partition protein	-
BB788_RS30805	18579..19013	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
BB788_RS30810	19010..19729	+	720	WP_001276217.1	plasmid SOS inhibition protein A	-
-	19741..19965	+	225	NuclAT_0	-	-
-	19741..19965	+	225	NuclAT_0	-	-
-	19741..19965	+	225	NuclAT_0	-	-
-	19741..19965	+	225	NuclAT_0	-	-
-	19898..19963	+	66	-	-	Antitoxin
BB788_RS30815	20009..20167	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
BB788_RS30820	20525..20950	+	426	WP_000422741.1	IS66 family insertion sequence hypothetical protein	-
BB788_RS30825	20947..21297	+	351	WP_000624722.1	IS66 family insertion sequence element accessory protein TnpB	-
BB788_RS30830	21328..22941	+	1614	WP_000080200.1	IS66-like element ISEc23 family transposase	-
BB788_RS32750	23026..23230	-	205	Protein_29	pilus protein	-
BB788_RS30850	23622..23918	+	297	WP_001272251.1	hypothetical protein	-
BB788_RS30855	24029..24850	+	822	WP_001234445.1	DUF945 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaCTX-M-55 / aac(6')-Ib-cr / blaOXA-1 / aac(3)-IIa	-	1..57266	57266

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T69774 WP_001312861.1 NZ_CP018343:20009-20167 [Klebsiella pneumoniae]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T69774 NZ_CP018343:20009-20167 [Klebsiella pneumoniae]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 66 bp

>AT69774 NZ_CP018343:19898-19963 [Klebsiella pneumoniae]
AGAAGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCAC

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin