Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 4452260..4452762 Replicon chromosome
Accession NZ_CP018323
Organism Escherichia coli strain KSC9

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag BS635_RS23055 Protein ID WP_000767829.1
Coordinates 4452260..4452514 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag BS635_RS23060 Protein ID WP_001259255.1
Coordinates 4452511..4452762 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BS635_RS23030 4447275..4447502 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
BS635_RS23035 4447516..4448574 - 1059 WP_000492325.1 transport protein YeeE -
BS635_RS23040 4448753..4450111 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
BS635_RS25490 4450101..4450163 - 63 WP_010723108.1 membrane protein YoeI -
BS635_RS23045 4450378..4451307 - 930 WP_000803351.1 LysR family transcriptional regulator -
BS635_RS23050 4451353..4452177 - 825 WP_000754737.1 SDR family oxidoreductase -
BS635_RS23055 4452260..4452514 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
BS635_RS23060 4452511..4452762 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
BS635_RS23065 4453045..4453095 + 51 WP_001364200.1 his operon leader peptide -
BS635_RS23075 4453241..4454140 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
BS635_RS23080 4454146..4455450 + 1305 WP_001295420.1 histidinol dehydrogenase -
BS635_RS23085 4455447..4456517 + 1071 WP_001099221.1 histidinol-phosphate transaminase -
BS635_RS23090 4456517..4457584 + 1068 WP_000080067.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T69746 WP_000767829.1 NZ_CP018323:c4452514-4452260 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T69746 NZ_CP018323:c4452514-4452260 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT69746 WP_001259255.1 NZ_CP018323:c4452762-4452511 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT69746 NZ_CP018323:c4452762-4452511 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References