Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 2608079..2608620 Replicon chromosome
Accession NZ_CP018323
Organism Escherichia coli strain KSC9

Toxin (Protein)


Gene name yafQ Uniprot ID E9TC49
Locus tag BS635_RS13395 Protein ID WP_000615982.1
Coordinates 2608079..2608357 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag BS635_RS13400 Protein ID WP_000729704.1
Coordinates 2608360..2608620 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BS635_RS13380 2605661..2606239 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
BS635_RS13385 2606445..2607212 + 768 WP_000333380.1 class II glutamine amidotransferase -
BS635_RS13390 2607183..2607923 - 741 WP_001225679.1 murein L,D-transpeptidase -
BS635_RS13395 2608079..2608357 - 279 WP_000615982.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
BS635_RS13400 2608360..2608620 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
BS635_RS13405 2608806..2609051 + 246 Protein_2429 peptidoglycan endopeptidase -
BS635_RS13410 2609029..2609421 + 393 WP_032260988.1 flagellar export chaperone FliS -
BS635_RS13415 2609426..2609737 + 312 WP_000414996.1 hypothetical protein -
BS635_RS13420 2609734..2610795 + 1062 WP_001067469.1 flagellar hook-length control protein FliK -
BS635_RS13425 2610803..2611270 + 468 WP_000725257.1 flagellar basal body-associated FliL family protein -
BS635_RS13430 2611290..2612006 + 717 WP_000938723.1 FliA/WhiG family RNA polymerase sigma factor -
BS635_RS13435 2612019..2612882 + 864 WP_001169518.1 flagellar motor stator protein MotA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10816.59 Da        Isoelectric Point: 10.0702

>T69731 WP_000615982.1 NZ_CP018323:c2608357-2608079 [Escherichia coli]
MIQRDIEYSGQFSKDVKVAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T69731 NZ_CP018323:c2608357-2608079 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAAGTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT69731 WP_000729704.1 NZ_CP018323:c2608620-2608360 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT69731 NZ_CP018323:c2608620-2608360 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References