Detailed information of TA system TA069727

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 1727852..1728454
• Replicon: chromosome
• Accession: NZ_CP018323
• Organism: Escherichia coli strain KSC9

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: BS635_RS08970
• Protein ID: WP_000897305.1
• Coordinates: 1727852..1728163 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: BS635_RS08975
• Protein ID: WP_000356397.1
• Coordinates: 1728164..1728454 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BS635_RS08945	1723766..1724365	+	600	WP_001295269.1	glucose-1-phosphatase	-
BS635_RS08950	1724359..1725231	+	873	WP_000920762.1	virulence factor BrkB family protein	-
BS635_RS08955	1725228..1725665	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
BS635_RS08960	1725710..1726651	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
BS635_RS08965	1726715..1727623	-	909	Protein_1627	alpha/beta hydrolase	-
BS635_RS08970	1727852..1728163	+	312	WP_000897305.1	hypothetical protein	Toxin
BS635_RS08975	1728164..1728454	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
BS635_RS08985	1729040..1729258	+	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
BS635_RS08990	1729477..1729719	+	243	WP_001087409.1	hypothetical protein	-
BS635_RS08995	1730049..1730978	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
BS635_RS09000	1730975..1731610	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
BS635_RS09005	1731607..1732509	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T69727 WP_000897305.1 NZ_CP018323:1727852-1728163 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T69727 NZ_CP018323:1727852-1728163 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT69727 WP_000356397.1 NZ_CP018323:1728164-1728454 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT69727 NZ_CP018323:1728164-1728454 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7