Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3298236..3298815 Replicon chromosome
Accession NZ_CP018319
Organism Pseudomonas frederiksbergensis strain AS1

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag PFAS1_RS15280 Protein ID WP_076029669.1
Coordinates 3298537..3298815 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag PFAS1_RS15275 Protein ID WP_076029668.1
Coordinates 3298236..3298523 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PFAS1_RS15245 3293644..3294072 + 429 WP_056744643.1 hypothetical protein -
PFAS1_RS15250 3294069..3294515 + 447 WP_076029663.1 DUF2442 domain-containing protein -
PFAS1_RS15255 3294570..3295649 - 1080 WP_076029664.1 tRNA (uridine(54)-C5)-methyltransferase TrmA -
PFAS1_RS15260 3295646..3296941 - 1296 WP_076029665.1 NCS2 family permease -
PFAS1_RS15265 3297156..3297611 + 456 WP_076029666.1 YolA family protein -
PFAS1_RS15270 3297678..3298229 + 552 WP_076029667.1 DJ-1/PfpI family protein -
PFAS1_RS15275 3298236..3298523 - 288 WP_076029668.1 HigA family addiction module antidote protein Antitoxin
PFAS1_RS15280 3298537..3298815 - 279 WP_076029669.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
PFAS1_RS15285 3298900..3300138 - 1239 WP_076029670.1 MFS transporter -
PFAS1_RS15290 3300623..3302062 + 1440 WP_076029671.1 cytochrome ubiquinol oxidase subunit I -
PFAS1_RS15295 3302066..3303073 + 1008 WP_076029672.1 cytochrome d ubiquinol oxidase subunit II -
PFAS1_RS15300 3303134..3303301 + 168 WP_074880353.1 DUF2474 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(1-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(20-60)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10750.29 Da        Isoelectric Point: 8.5480

>T69718 WP_076029669.1 NZ_CP018319:c3298815-3298537 [Pseudomonas frederiksbergensis]
MIKSFQHKGLRGFYETGSTRGIRADHAKRLSRMLQFMDHAAVPGDLDIPGWRLHPLRGELTEYWSLSVSGNWRIIFRFIG
SDIELVDYLDYH

Download         Length: 279 bp

>T69718 NZ_CP018319:c3298815-3298537 [Pseudomonas frederiksbergensis]
ATGATCAAATCCTTTCAGCACAAAGGCCTTCGTGGCTTCTATGAAACAGGTTCGACTCGCGGGATCCGGGCAGACCATGC
GAAGCGTCTGTCTCGAATGCTTCAGTTCATGGACCACGCAGCAGTACCTGGCGACCTTGACATTCCGGGATGGCGATTAC
ACCCACTCAGGGGAGAGTTGACCGAATACTGGTCACTGAGCGTTTCGGGGAACTGGCGAATTATTTTTCGTTTTATCGGT
TCGGATATCGAACTCGTCGACTATCTGGACTACCACTGA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10591.20 Da        Isoelectric Point: 6.6041

>AT69718 WP_076029668.1 NZ_CP018319:c3298523-3298236 [Pseudomonas frederiksbergensis]
MPMHNPPHPGETLLMDVLPELGVSVTELAQHLRFARPHLSRVLHGHAPISPDLAVRLERAGIGKARMWLGAQTDYDLWQA
EQREQPVIEPIAAHA

Download         Length: 288 bp

>AT69718 NZ_CP018319:c3298523-3298236 [Pseudomonas frederiksbergensis]
ATGCCCATGCACAACCCGCCACACCCTGGCGAAACCCTTTTGATGGATGTATTGCCTGAACTTGGCGTCAGCGTCACAGA
GCTGGCCCAGCACCTGCGATTTGCGCGTCCGCATCTATCGCGAGTGCTGCATGGCCACGCGCCGATCAGCCCTGATTTGG
CGGTACGCCTGGAGCGGGCTGGAATTGGTAAGGCTCGAATGTGGCTAGGTGCTCAGACTGACTACGATCTCTGGCAAGCA
GAGCAGCGTGAGCAACCGGTCATCGAACCCATTGCCGCCCACGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References