Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 2876782..2877379 Replicon chromosome
Accession NZ_CP018307
Organism Klebsiella variicola strain X39

Toxin (Protein)


Gene name higB Uniprot ID A0A1F2LYJ8
Locus tag BC497_RS14225 Protein ID WP_023322191.1
Coordinates 2877062..2877379 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A6H0BIE0
Locus tag BC497_RS14220 Protein ID WP_023322192.1
Coordinates 2876782..2877069 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BC497_RS14185 2872692..2872940 + 249 WP_008805539.1 DUF1158 domain-containing protein -
BC497_RS14190 2872957..2873298 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
BC497_RS14195 2873329..2874444 - 1116 WP_162493283.1 MBL fold metallo-hydrolase -
BC497_RS14200 2874624..2875205 + 582 WP_087653534.1 TetR/AcrR family transcriptional regulator -
BC497_RS14205 2875205..2875573 + 369 WP_008805536.1 MmcQ/YjbR family DNA-binding protein -
BC497_RS14210 2875693..2876346 + 654 WP_087653533.1 oxygen-insensitive NAD(P)H nitroreductase -
BC497_RS14220 2876782..2877069 - 288 WP_023322192.1 helix-turn-helix transcriptional regulator Antitoxin
BC497_RS14225 2877062..2877379 - 318 WP_023322191.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
BC497_RS14230 2877564..2878607 - 1044 WP_023339753.1 DUF2157 domain-containing protein -
BC497_RS14240 2879286..2880152 - 867 WP_008805530.1 helix-turn-helix transcriptional regulator -
BC497_RS14245 2880261..2881688 + 1428 WP_023297179.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-89)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T69696 WP_023322191.1 NZ_CP018307:c2877379-2877062 [Klebsiella variicola]
MFRIVVHVDVKKELQALPPIVQAKMIRQIDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T69696 NZ_CP018307:c2877379-2877062 [Klebsiella variicola]
ATGTTCAGAATAGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAAATAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10801.48 Da        Isoelectric Point: 10.2212

>AT69696 WP_023322192.1 NZ_CP018307:c2877069-2876782 [Klebsiella variicola]
MSKKIIDWDTLKTELLSQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANVTRASLDTLTR
YALACGIKHPQILLY

Download         Length: 288 bp

>AT69696 NZ_CP018307:c2877069-2876782 [Klebsiella variicola]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTAGCCAGCCTGAGGTTCAGGCTGCGTTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTGACCCGCGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCGACGGTCTCCAGAATGGAAGCCAATGTCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGTGGAATAAAGCACCCACAAATTTTACTTTACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1F2LYJ8

Antitoxin

Source ID Structure
AlphaFold DB A0A6H0BIE0

References