Detailed information of TA system TA069676

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 3185626..3186223
• Replicon: chromosome
• Accession: NZ_CP018306
• Organism: Klebsiella pneumoniae strain Klebsiella pneumoniae 459 isolate 459

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A9J6S5A1
• Locus tag: A9493_RS15990
• Protein ID: WP_004893639.1
• Coordinates: 3185626..3185943 (+)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: R4YH91
• Locus tag: A9493_RS15995
• Protein ID: WP_004142561.1
• Coordinates: 3185936..3186223 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A9493_RS15970	3181326..3182753	-	1428	WP_004176980.1	MFS transporter	-
A9493_RS15975	3182862..3183728	+	867	WP_004151823.1	helix-turn-helix transcriptional regulator	-
A9493_RS15985	3184398..3185441	+	1044	WP_023279204.1	DUF2157 domain-containing protein	-
A9493_RS15990	3185626..3185943	+	318	WP_004893639.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
A9493_RS15995	3185936..3186223	+	288	WP_004142561.1	helix-turn-helix transcriptional regulator	Antitoxin
A9493_RS16000	3186361..3187014	-	654	WP_004178896.1	oxygen-insensitive NAD(P)H nitroreductase	-
A9493_RS16005	3187134..3187502	-	369	WP_004142557.1	MmcQ/YjbR family DNA-binding protein	-
A9493_RS16010	3187502..3188083	-	582	WP_004176968.1	TetR/AcrR family transcriptional regulator	-
A9493_RS16015	3188263..3189378	+	1116	WP_012737592.1	MBL fold metallo-hydrolase	-
A9493_RS16020	3189409..3189750	+	342	WP_002893035.1	RamA family antibiotic efflux transcriptional regulator	-
A9493_RS16025	3189768..3190016	-	249	WP_002893037.1	DUF1158 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-88)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12100.35 Da
• Isoelectric Point: 11.2767

>T69676 WP_004893639.1 NZ_CP018306:3185626-3185943 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPSIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Nucleotide

• Length: 318 bp

>T69676 NZ_CP018306:3185626-3185943 [Klebsiella pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGTCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10771.46 Da
• Isoelectric Point: 10.2212

>AT69676 WP_004142561.1 NZ_CP018306:3185936-3186223 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Nucleotide

• Length: 288 bp

>AT69676 NZ_CP018306:3185936-3186223 [Klebsiella pneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCGACGGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACGTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Structures

Antitoxin

• AlphaFold DB: A0A0S3DIQ1