Detailed information of TA system TA069645

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 2249940..2250859
• Replicon: chromosome
• Accession: NZ_CP018305
• Organism: Mycobacterium tuberculosis strain M0018684-2

Toxin (Protein)

• Gene name: HigB2
• Uniprot ID: A0A654U351
• Locus tag: BOM22_RS10935
• Protein ID: WP_003906719.1
• Coordinates: 2250254..2250859 (-)
• Length: 202 a.a.

Antitoxin (Protein)

• Gene name: HigA2
• Uniprot ID: L7N5K9
• Locus tag: BOM22_RS10930
• Protein ID: WP_003410124.1
• Coordinates: 2249940..2250245 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BOM22_RS10900	2244951..2246207	-	1257	WP_003410095.1	HNH endonuclease	-
BOM22_RS10905	2246561..2247136	+	576	WP_003410100.1	hypothetical protein	-
BOM22_RS10910	2247133..2248173	+	1041	WP_003410103.1	ImmA/IrrE family metallo-endopeptidase	-
BOM22_RS10915	2248415..2249134	+	720	WP_003410108.1	DUF433 domain-containing protein	-
BOM22_RS10920	2249124..2249540	+	417	WP_003410114.1	hypothetical protein	-
BOM22_RS10925	2249556..2249855	-	300	WP_003410120.1	hypothetical protein	-
BOM22_RS10930	2249940..2250245	-	306	WP_003410124.1	XRE family transcriptional regulator	Antitoxin
BOM22_RS10935	2250254..2250859	-	606	WP_003906719.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BOM22_RS10940	2250884..2251243	-	360	WP_003410131.1	hypothetical protein	-
BOM22_RS10945	2251403..2252080	-	678	WP_003900450.1	hypothetical protein	-
BOM22_RS10950	2252032..2252268	-	237	WP_003410133.1	hypothetical protein	-
BOM22_RS10955	2252475..2253371	-	897	WP_003906720.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (86-181)

Antitoxin

• HigA2-like, helix-turn-helix domain (32-92)

Sequences

Toxin

Protein

• Length: 202 a.a.
• Molecular weight: 22716.91 Da
• Isoelectric Point: 7.3073

>T69645 WP_003906719.1 NZ_CP018305:c2250859-2250254 [Mycobacterium tuberculosis]
VNVPWENAHGGALYCLIRGDEFSAWHRLLFQRPGCAESVLACRHFLDGSPVARCSYPEEYHPCVISRIALLCDSVGWTAD
VERISAWLNGLDRETYELVFAAIEVLEEEGPALGCPLADTVRGSRHKNMKELRPGSQGRSEVRILFAFDPARQAIMLAAG
NKAGRWTQWYDEKIKAADEMFAEHLAQFEDTKPKRRKRKKG

Nucleotide

• Length: 606 bp

>T69645 NZ_CP018305:c2250859-2250254 [Mycobacterium tuberculosis]
GTGAACGTTCCATGGGAGAACGCGCACGGCGGCGCACTGTACTGCCTCATACGTGGGGATGAATTCTCGGCCTGGCACAG
GCTACTGTTTCAACGGCCCGGGTGCGCAGAGTCCGTCCTCGCCTGCCGGCATTTCCTCGACGGTAGCCCCGTCGCCCGCT
GCTCTTACCCGGAGGAATATCACCCTTGTGTGATATCGCGTATCGCGCTACTCTGCGACTCTGTGGGGTGGACGGCGGAT
GTGGAGCGCATCTCCGCCTGGCTCAACGGCTTGGACCGAGAGACCTACGAATTGGTGTTCGCAGCTATCGAGGTTCTCGA
GGAAGAGGGCCCCGCACTTGGCTGTCCTCTGGCGGACACGGTGCGTGGGTCGCGCCACAAGAACATGAAGGAGCTGCGTC
CAGGATCACAAGGGCGGTCCGAGGTCCGCATTCTGTTCGCCTTCGACCCGGCGCGGCAGGCGATCATGCTGGCCGCCGGT
AACAAGGCCGGGCGGTGGACCCAGTGGTACGACGAGAAGATCAAAGCGGCCGATGAGATGTTTGCCGAACATCTCGCACA
GTTCGAGGACACGAAGCCAAAGAGGCGCAAGCGCAAGAAAGGGTAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11291.91 Da
• Isoelectric Point: 10.7482

>AT69645 WP_003410124.1 NZ_CP018305:c2250245-2249940 [Mycobacterium tuberculosis]
MAMTLRDMDAVRPVNREAVDRHKARMRDEVRAFRLRELRAAQSLTQVQVAALAHIRQSRVSSIENGDIGSAQVNTLRKYV
SALGGELDITVRLGDETFTLA

Nucleotide

• Length: 306 bp

>AT69645 NZ_CP018305:c2250245-2249940 [Mycobacterium tuberculosis]
ATGGCGATGACACTACGGGACATGGACGCCGTACGTCCCGTTAACCGGGAGGCGGTGGACCGGCACAAGGCACGAATGCG
CGATGAAGTGCGGGCATTCCGGCTCCGGGAGCTGCGCGCGGCTCAGTCACTCACCCAGGTCCAGGTGGCTGCGCTGGCGC
ACATCCGGCAGAGCCGGGTATCCAGCATCGAGAACGGCGACATCGGCTCCGCACAGGTCAACACACTGCGCAAATACGTG
TCCGCTCTTGGCGGAGAACTGGATATCACCGTCCGCCTGGGAGATGAGACATTCACCCTGGCATAG

Structures

Toxin

• AlphaFold DB: A0A654U351

Antitoxin

• AlphaFold DB: A0A7U4BV30