Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) unclassified/-
Location 2960728..2961376 Replicon chromosome
Accession NZ_CP018302
Organism Mycobacterium tuberculosis strain I0004000-1

Toxin (Protein)


Gene name novel[antitoxin] Uniprot ID P9WJ12
Locus tag BOJ12_RS14460 Protein ID WP_003899414.1
Coordinates 2960728..2961051 (-) Length 108 a.a.

Antitoxin (Protein)


Gene name novel[toxin] Uniprot ID P9WJ10
Locus tag BOJ12_RS14465 Protein ID WP_003899415.1
Coordinates 2961131..2961376 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BOJ12_RS14440 2956389..2957650 + 1262 WP_087902221.1 IS3-like element IS987 family transposase -
BOJ12_RS14445 2957937..2959376 - 1440 WP_003899411.1 phage major capsid protein -
BOJ12_RS14450 2959384..2959917 - 534 WP_003899412.1 HK97 family phage prohead protease -
BOJ12_RS14455 2960070..2960561 - 492 WP_003900541.1 phage terminase small subunit P27 family -
BOJ12_RS14460 2960728..2961051 - 324 WP_003899414.1 type II toxin-antitoxin system toxin Toxin
BOJ12_RS14465 2961131..2961376 - 246 WP_003899415.1 type II toxin-antitoxin system antitoxin Antitoxin
BOJ12_RS14470 2961373..2962800 - 1428 WP_003899416.1 DUF3631 domain-containing protein -
BOJ12_RS14475 2962802..2963194 - 393 WP_003899417.1 DUF2742 domain-containing protein -
BOJ12_RS14480 2963191..2963451 - 261 WP_003899418.1 helix-turn-helix domain-containing protein -
BOJ12_RS14485 2963468..2963830 - 363 WP_003900543.1 hypothetical protein -
BOJ12_RS14490 2963833..2964960 - 1128 WP_003899420.1 site-specific integrase -
BOJ12_RS14495 2965105..2965332 - 228 WP_003899421.1 hypothetical protein -
BOJ12_RS14500 2965329..2965718 - 390 WP_003899422.1 hypothetical protein -
BOJ12_RS14505 2965624..2965896 + 273 WP_003900544.1 hypothetical protein -
BOJ12_RS14510 2965995..2966228 + 234 WP_003413717.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 2954693..2964960 10267


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(5-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(16-65)


Sequences

Toxin        


Download         Length: 108 a.a.        Molecular weight: 12359.82 Da        Isoelectric Point: 8.6717

>T69472 WP_003899414.1 NZ_CP018302:c2961051-2960728 [Mycobacterium tuberculosis]
MTHKRTKRQPAIAAGLNAPRRNRVGRQHGWPADVPSAEQRRAQRQRDLEAIRRAYAEMVATSHEIDDDTAELALLSMHLD
DEQRRLEAGMKLGWHPYHFPDEPDSKQ

Download         Length: 324 bp

>T69472 NZ_CP018302:c2961051-2960728 [Mycobacterium tuberculosis]
TTGACCCACAAGCGCACTAAACGCCAGCCAGCCATCGCCGCAGGGCTCAACGCCCCGCGTCGGAATCGCGTTGGGCGGCA
ACATGGTTGGCCGGCCGACGTTCCGTCCGCCGAGCAGCGCCGCGCCCAACGGCAGCGCGACCTCGAGGCTATCCGCCGAG
CGTACGCCGAGATGGTGGCGACATCACACGAAATCGACGACGACACAGCCGAACTGGCGCTGTTGTCGATGCATCTCGAC
GATGAGCAGCGCCGGCTTGAGGCGGGGATGAAGCTCGGCTGGCATCCGTATCACTTCCCCGACGAACCCGACAGCAAACA
GTGA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 7697.71 Da        Isoelectric Point: 4.8565

>AT69472 WP_003899415.1 NZ_CP018302:c2961376-2961131 [Mycobacterium tuberculosis]
MSGHALAARTLLAAADELVGGPPVEASAAALAGDAAGAWRTAAVELARALVRAVAESHGVAAVLFAATAAAAAAVDRGDP
P

Download         Length: 246 bp

>AT69472 NZ_CP018302:c2961376-2961131 [Mycobacterium tuberculosis]
ATGAGCGGCCACGCGTTGGCTGCTCGGACGTTGCTGGCCGCCGCGGACGAGCTTGTCGGCGGCCCGCCAGTCGAGGCTTC
GGCCGCCGCGCTGGCCGGCGACGCCGCGGGCGCATGGCGGACCGCGGCCGTCGAGCTTGCGCGAGCGTTGGTCCGCGCTG
TGGCGGAGTCGCACGGCGTCGCGGCCGTTTTGTTCGCCGCGACGGCCGCCGCGGCGGCGGCCGTCGACCGGGGTGATCCG
CCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A045IHC4

Antitoxin

Source ID Structure
AlphaFold DB A0A806JR81

References