Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 3531101..3531771 Replicon chromosome
Accession NZ_CP018301
Organism Mycobacterium tuberculosis strain I0002801-4

Toxin (Protein)


Gene name higB Uniprot ID O53332
Locus tag BOJ61_RS17300 Protein ID WP_003899954.1
Coordinates 3531101..3531445 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0THF6
Locus tag BOJ61_RS17305 Protein ID WP_003899955.1
Coordinates 3531442..3531771 (+) Length 110 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BOJ61_RS17265 3526174..3527034 + 861 WP_003416628.1 alpha/beta hydrolase -
BOJ61_RS17270 3527009..3527524 + 516 WP_003899950.1 nitroreductase family deazaflavin-dependent oxidoreductase -
BOJ61_RS17280 3527764..3528057 + 294 WP_003416635.1 hypothetical protein -
BOJ61_RS17285 3528345..3529634 + 1290 WP_003416640.1 ATP-binding protein -
BOJ61_RS17290 3529981..3530415 - 435 WP_003899952.1 type II toxin-antitoxin system VapC family toxin -
BOJ61_RS17295 3530418..3530870 - 453 WP_003899953.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
BOJ61_RS17300 3531101..3531445 + 345 WP_003899954.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
BOJ61_RS17305 3531442..3531771 + 330 WP_003899955.1 XRE family transcriptional regulator Antitoxin
BOJ61_RS17310 3532309..3532656 + 348 WP_003899956.1 DUF2384 domain-containing protein -
BOJ61_RS17315 3532653..3533273 + 621 WP_003899957.1 RES family NAD+ phosphorylase -
BOJ61_RS17320 3533433..3534698 - 1266 WP_003899958.1 hypothetical protein -
BOJ61_RS17325 3534866..3535075 + 210 WP_003416778.1 hypothetical protein -
BOJ61_RS17335 3535322..3536356 - 1035 WP_003416786.1 IS30 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-91)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12692.50 Da        Isoelectric Point: 5.6920

>T69420 WP_003899954.1 NZ_CP018301:3531101-3531445 [Mycobacterium tuberculosis]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG

Download         Length: 345 bp

>T69420 NZ_CP018301:3531101-3531445 [Mycobacterium tuberculosis]
TTGGCCGTGATCCTGCTCCCGCAGGTCGAACGGTGGTTCTTCGCGCTCAACAGGGATGCGATGGCCTCGGTCACCGGCGC
CATCGACCTGCTCGAAATGGAGGGGCCGACGTTGGGCCGCCCGGTGGTCGACAAAGTGAACGACTCAACGTTTCACAACA
TGAAGGAGCTGCGCCCCGCCGGCACCAGCATCCGGATCCTGTTCGCCTTCGACCCGGCCCGGCAGGCGATCCTGCTGCTG
GGCGGTGACAAGGCAGGCAACTGGAAACGCTGGTACGACAACAACATTCCAATCGCTGACCAGCGCTCCGAGAACTGGCT
GGCGAGCGAGCACGGAGGTGGATGA

Antitoxin


Download         Length: 110 a.a.        Molecular weight: 11802.46 Da        Isoelectric Point: 7.4051

>AT69420 WP_003899955.1 NZ_CP018301:3531442-3531771 [Mycobacterium tuberculosis]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA

Download         Length: 330 bp

>AT69420 NZ_CP018301:3531442-3531771 [Mycobacterium tuberculosis]
ATGACCATGGCCCGCAACTGGCGTGACATTCGCGCCGATGCCGTCGCGCAGGGCCGCGTGGATCTGCAGCGGGCCGCCGT
GGCACGCGAGGAGATGCGCGATGCCGTCCTGGCGCACCGCCTGGCCGAGATCCGCAAGGCGCTAGGCCACGCACGTCAGG
CCGACGTCGCGGCGCTGATGGGGGTCTCTCAGGCCCGTGTCTCCAAGCTGGAGAGCGGCGACCTGTCCCACACCGAACTC
GGCACCCTGCAGGCCTACGTTGCCGCCCTGGGCGGGCACCTGCGCATCGTCGCTGAGTTCGGCGAAAATACTGTCGAGCT
GACCGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FBK2

Antitoxin

Source ID Structure
PDB 6LTY
PDB 6LTZ
AlphaFold DB G0THF6

References