Detailed information of TA system TA069359

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 3537045..3537715
• Replicon: chromosome
• Accession: NZ_CP018300
• Organism: Mycobacterium tuberculosis strain I0002353-6

Toxin (Protein)

• Gene name: higB
• Uniprot ID: O53332
• Locus tag: BOM19_RS17365
• Protein ID: WP_003899954.1
• Coordinates: 3537045..3537389 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: G0THF6
• Locus tag: BOM19_RS17370
• Protein ID: WP_003899955.1
• Coordinates: 3537386..3537715 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BOM19_RS17330	3532118..3532978	+	861	WP_003416628.1	alpha/beta hydrolase	-
BOM19_RS17335	3532953..3533468	+	516	WP_003899950.1	nitroreductase family deazaflavin-dependent oxidoreductase	-
BOM19_RS17340	3533484..3533695	+	212	Protein_3274	(R)-hydratase	-
BOM19_RS17345	3533708..3534001	+	294	WP_003416635.1	hypothetical protein	-
BOM19_RS17350	3534289..3535578	+	1290	WP_003906996.1	ATP-binding protein	-
BOM19_RS17355	3535925..3536359	-	435	WP_003899952.1	type II toxin-antitoxin system VapC family toxin	-
BOM19_RS17360	3536362..3536814	-	453	WP_003899953.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
BOM19_RS17365	3537045..3537389	+	345	WP_003899954.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BOM19_RS17370	3537386..3537715	+	330	WP_003899955.1	XRE family transcriptional regulator	Antitoxin
BOM19_RS17375	3538253..3538600	+	348	WP_003899956.1	DUF2384 domain-containing protein	-
BOM19_RS17380	3538597..3539217	+	621	WP_003899957.1	RES family NAD+ phosphorylase	-
BOM19_RS17385	3539377..3540642	-	1266	WP_023641391.1	hypothetical protein	-
BOM19_RS17390	3540810..3541019	+	210	WP_003416778.1	hypothetical protein	-
BOM19_RS17400	3541266..3542300	-	1035	WP_003416786.1	IS30 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-91)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 12692.50 Da
• Isoelectric Point: 5.6920

>T69359 WP_003899954.1 NZ_CP018300:3537045-3537389 [Mycobacterium tuberculosis]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG

Nucleotide

• Length: 345 bp

>T69359 NZ_CP018300:3537045-3537389 [Mycobacterium tuberculosis]
TTGGCCGTGATCCTGCTCCCGCAGGTCGAACGGTGGTTCTTCGCGCTCAACAGGGATGCGATGGCCTCGGTCACCGGCGC
CATCGACCTGCTCGAAATGGAGGGGCCGACGTTGGGCCGCCCGGTGGTCGACAAAGTGAACGACTCAACGTTTCACAACA
TGAAGGAGCTGCGCCCCGCCGGCACCAGCATCCGGATCCTGTTCGCCTTCGACCCGGCCCGGCAGGCGATCCTGCTGCTG
GGCGGTGACAAGGCAGGCAACTGGAAACGCTGGTACGACAACAACATTCCAATCGCTGACCAGCGCTCCGAGAACTGGCT
GGCGAGCGAGCACGGAGGTGGATGA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11802.46 Da
• Isoelectric Point: 7.4051

>AT69359 WP_003899955.1 NZ_CP018300:3537386-3537715 [Mycobacterium tuberculosis]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA

Nucleotide

• Length: 330 bp

>AT69359 NZ_CP018300:3537386-3537715 [Mycobacterium tuberculosis]
ATGACCATGGCCCGCAACTGGCGTGACATTCGCGCCGATGCCGTCGCGCAGGGCCGCGTGGATCTGCAGCGGGCCGCCGT
GGCACGCGAGGAGATGCGCGATGCCGTCCTGGCGCACCGCCTGGCCGAGATCCGCAAGGCGCTAGGCCACGCACGTCAGG
CCGACGTCGCGGCGCTGATGGGGGTCTCTCAGGCCCGTGTCTCCAAGCTGGAGAGCGGCGACCTGTCCCACACCGAACTC
GGCACCCTGCAGGCCTACGTTGCCGCCCTGGGCGGGCACCTGCGCATCGTCGCTGAGTTCGGCGAAAATACTGTCGAGCT
GACCGCCTGA

Structures

Toxin

• AlphaFold DB: A0A7U4FBK2

Antitoxin

• AlphaFold DB: G0THF6