Detailed information of TA system TA069175

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 1205903..1206128
• Replicon: chromosome
• Accession: NZ_CP018247
• Organism: Escherichia coli strain 7784

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: -
• Locus tag: BFL18_RS06185
• Protein ID: WP_000813263.1
• Coordinates: 1205973..1206128 (+)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	1205903..1205961 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BFL18_RS06160	1201160..1202269	+	1110	WP_021499850.1	putative primosomal protein I	-
BFL18_RS06165	1202276..1203022	+	747	WP_000788751.1	ATP-binding protein	-
BFL18_RS06170	1203044..1203814	+	771	WP_000450992.1	DUF1627 domain-containing protein	-
BFL18_RS06175	1203830..1204243	+	414	WP_001151233.1	DUF977 family protein	-
BFL18_RS06180	1204595..1205368	-	774	WP_000160654.1	alpha/beta hydrolase	-
-	1205903..1205961	-	59	-	-	Antitoxin
BFL18_RS06185	1205973..1206128	+	156	WP_000813263.1	type I toxin-antitoxin system toxin HokD	Toxin
BFL18_RS06190	1206296..1206574	+	279	WP_001341388.1	hypothetical protein	-
BFL18_RS06195	1206576..1207625	+	1050	WP_001265172.1	DUF968 domain-containing protein	-
BFL18_RS06200	1207638..1208009	+	372	WP_001217436.1	RusA family crossover junction endodeoxyribonuclease	-
BFL18_RS06205	1207999..1208370	+	372	WP_000090264.1	antitermination protein	-
BFL18_RS06210	1208522..1209340	+	819	WP_000265265.1	CPBP family intramembrane metalloprotease	-
BFL18_RS06215	1209627..1209823	+	197	Protein_1111	TrmB family transcriptional regulator	-
BFL18_RS06220	1209961..1210674	+	714	WP_000261909.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5722.86 Da
• Isoelectric Point: 6.1531

>T69175 WP_000813263.1 NZ_CP018247:1205973-1206128 [Escherichia coli]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRVRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T69175 NZ_CP018247:1205973-1206128 [Escherichia coli]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAGTCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT69175 NZ_CP018247:c1205961-1205903 [Escherichia coli]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTAGATTTGCCGTTCATAGATTGAGCCTCA

Structures

Antitoxin