Detailed information of TA system TA069140

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 292447..293141
• Replicon: chromosome
• Accession: NZ_CP018245
• Organism: Escherichia coli strain 472

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: C3TNX7
• Locus tag: BFL23_RS01440
• Protein ID: WP_001263495.1
• Coordinates: 292743..293141 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: BFL23_RS01435
• Protein ID: WP_000554757.1
• Coordinates: 292447..292740 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BFL23_RS31480	288158..288655	+	498	Protein_252	REP-associated tyrosine transposase RayT	-
BFL23_RS01420	288800..290512	-	1713	Protein_253	flagellar biosynthesis protein FlhA	-
BFL23_RS01425	290484..291269	+	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
BFL23_RS01430	291340..292395	+	1056	WP_001226186.1	DNA polymerase IV	-
BFL23_RS01435	292447..292740	+	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
BFL23_RS01440	292743..293141	+	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
BFL23_RS01445	293151..293603	+	453	WP_001059871.1	GNAT family N-acetyltransferase	-
BFL23_RS01450	293922..294125	+	204	Protein_259	RNA ligase RtcB family protein	-
BFL23_RS01455	294124..294645	+	522	Protein_260	peptide chain release factor H	-
BFL23_RS01460	294702..296159	-	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
BFL23_RS01465	296420..296878	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
-	297474..297554	+	81	NuclAT_12	-	-
-	297474..297554	+	81	NuclAT_12	-	-
-	297474..297554	+	81	NuclAT_12	-	-
-	297474..297554	+	81	NuclAT_12	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.86 Da
• Isoelectric Point: 8.0949

>T69140 WP_001263495.1 NZ_CP018245:292743-293141 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T69140 NZ_CP018245:292743-293141 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAGCTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACTTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAATCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT69140 WP_000554757.1 NZ_CP018245:292447-292740 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT69140 NZ_CP018245:292447-292740 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAATTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: S1FJN6