Detailed information of TA system TA069108

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 1721998..1722223
• Replicon: chromosome
• Accession: NZ_CP018243
• Organism: Escherichia coli strain 350

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: A0A1Q4PF16
• Locus tag: BFL21_RS09425
• Protein ID: WP_000813258.1
• Coordinates: 1722068..1722223 (+)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	1721998..1722056 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BFL21_RS09380	1717052..1717279	+	228	WP_000912298.1	transcriptional regulator	-
BFL21_RS09385	1717263..1717814	+	552	WP_000705621.1	hypothetical protein	-
BFL21_RS30165	1717786..1718826	+	1041	WP_140425393.1	DNA-binding protein	-
BFL21_RS30885	1718738..1719280	+	543	WP_072130322.1	replication protein	-
BFL21_RS09395	1719314..1720030	+	717	WP_000450627.1	DUF1627 domain-containing protein	-
BFL21_RS09400	1720063..1720344	+	282	WP_000603384.1	DNA-binding protein	-
BFL21_RS09405	1720341..1720568	+	228	WP_000699809.1	hypothetical protein	-
BFL21_RS09410	1720561..1720872	+	312	WP_001289673.1	hypothetical protein	-
BFL21_RS09415	1721000..1721218	+	219	WP_000683609.1	DUF4014 family protein	-
BFL21_RS09420	1721220..1721777	+	558	WP_000104474.1	DUF551 domain-containing protein	-
-	1721998..1722056	-	59	-	-	Antitoxin
BFL21_RS09425	1722068..1722223	+	156	WP_000813258.1	type I toxin-antitoxin system Hok family toxin	Toxin
BFL21_RS09430	1722343..1722687	+	345	WP_000756596.1	YgiW/YdeI family stress tolerance OB fold protein	-
BFL21_RS09435	1722809..1723081	+	273	WP_000191872.1	hypothetical protein	-
BFL21_RS09440	1723083..1724132	+	1050	WP_001265229.1	DUF968 domain-containing protein	-
BFL21_RS09445	1724145..1724450	+	306	WP_001217444.1	RusA family crossover junction endodeoxyribonuclease	-
BFL21_RS09450	1724513..1725067	+	555	WP_000640035.1	DUF1133 family protein	-
BFL21_RS30890	1725292..1725489	+	198	WP_000917763.1	hypothetical protein	-
BFL21_RS09460	1725625..1726338	+	714	WP_000301797.1	helix-turn-helix domain-containing protein	-
BFL21_RS09475	1726789..1727220	+	432	WP_001302123.1	tellurite resistance protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1706018..1759331	53313

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5767.95 Da
• Isoelectric Point: 7.7942

>T69108 WP_000813258.1 NZ_CP018243:1722068-1722223 [Escherichia coli]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFVDYESEK

Nucleotide

• Length: 156 bp

>T69108 NZ_CP018243:1722068-1722223 [Escherichia coli]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTGCGAATCCGAACCGGTCAGACGGAGGTCGCTGTCTTCGTAGACTACGAATCTGAGAAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT69108 NZ_CP018243:c1722056-1721998 [Escherichia coli]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Toxin

• AlphaFold DB: A0A1Q4PF16

Antitoxin