Detailed information of TA system TA069103

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 1240956..1241181
• Replicon: chromosome
• Accession: NZ_CP018243
• Organism: Escherichia coli strain 350

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: -
• Locus tag: BFL21_RS06270
• Protein ID: WP_000813263.1
• Coordinates: 1241026..1241181 (+)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	1240956..1241014 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BFL21_RS06245	1236231..1237322	+	1092	WP_001205823.1	hypothetical protein	-
BFL21_RS06250	1237329..1238075	+	747	WP_000788751.1	ATP-binding protein	-
BFL21_RS06255	1238097..1238867	+	771	WP_000450992.1	DUF1627 domain-containing protein	-
BFL21_RS06260	1238883..1239296	+	414	WP_001151233.1	DUF977 family protein	-
BFL21_RS06265	1239648..1240421	-	774	WP_000160654.1	alpha/beta hydrolase	-
-	1240956..1241014	-	59	-	-	Antitoxin
BFL21_RS06270	1241026..1241181	+	156	WP_000813263.1	type I toxin-antitoxin system toxin HokD	Toxin
BFL21_RS06275	1241349..1241627	+	279	WP_001341388.1	hypothetical protein	-
BFL21_RS06280	1241629..1242678	+	1050	WP_001265172.1	DUF968 domain-containing protein	-
BFL21_RS06285	1242691..1243062	+	372	WP_001217436.1	RusA family crossover junction endodeoxyribonuclease	-
BFL21_RS06290	1243052..1243423	+	372	WP_000090264.1	antitermination protein	-
BFL21_RS06295	1243575..1244393	+	819	WP_000265265.1	CPBP family intramembrane metalloprotease	-
BFL21_RS06300	1244680..1244876	+	197	Protein_1117	TrmB family transcriptional regulator	-
BFL21_RS06305	1245014..1245727	+	714	WP_000261909.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5722.86 Da
• Isoelectric Point: 6.1531

>T69103 WP_000813263.1 NZ_CP018243:1241026-1241181 [Escherichia coli]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRVRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T69103 NZ_CP018243:1241026-1241181 [Escherichia coli]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAGTCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT69103 NZ_CP018243:c1241014-1240956 [Escherichia coli]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTAGATTTGCCGTTCATAGATTGAGCCTCA

Structures

Antitoxin