Detailed information of TA system TA069065

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 1812444..1812669
• Replicon: chromosome
• Accession: NZ_CP018241
• Organism: Escherichia coli strain 319

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: A0A1Q4PF16
• Locus tag: BFL24_RS09925
• Protein ID: WP_000813258.1
• Coordinates: 1812514..1812669 (+)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	1812444..1812502 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BFL24_RS09870	1807952..1808413	+	462	WP_000139447.1	replication protein	-
BFL24_RS09875	1808447..1809163	+	717	WP_000450627.1	DUF1627 domain-containing protein	-
BFL24_RS09880	1809196..1809465	+	270	WP_072640384.1	DNA-binding protein	-
BFL24_RS09895	1809471..1810684	+	1214	WP_162829202.1	IS3 family transposase	-
BFL24_RS09905	1810787..1811014	+	228	WP_000699809.1	hypothetical protein	-
BFL24_RS09910	1811007..1811318	+	312	WP_001289673.1	hypothetical protein	-
BFL24_RS09915	1811446..1811664	+	219	WP_000683609.1	DUF4014 family protein	-
BFL24_RS09920	1811666..1812223	+	558	WP_000104474.1	DUF551 domain-containing protein	-
-	1812444..1812502	-	59	-	-	Antitoxin
BFL24_RS09925	1812514..1812669	+	156	WP_000813258.1	type I toxin-antitoxin system Hok family toxin	Toxin
BFL24_RS09930	1812789..1813133	+	345	WP_000756596.1	YgiW/YdeI family stress tolerance OB fold protein	-
BFL24_RS09935	1813255..1813527	+	273	WP_000191872.1	hypothetical protein	-
BFL24_RS09940	1813529..1814578	+	1050	WP_001265229.1	DUF968 domain-containing protein	-
BFL24_RS09945	1814591..1814896	+	306	WP_001217444.1	RusA family crossover junction endodeoxyribonuclease	-
BFL24_RS09950	1814959..1815513	+	555	WP_000640035.1	DUF1133 family protein	-
BFL24_RS31120	1815738..1815935	+	198	WP_000917763.1	hypothetical protein	-
BFL24_RS09960	1816071..1816784	+	714	WP_000301797.1	helix-turn-helix domain-containing protein	-
BFL24_RS09975	1817235..1817666	+	432	WP_001302123.1	tellurite resistance protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	paa / nleG7'	1748148..1849773	101625

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5767.95 Da
• Isoelectric Point: 7.7942

>T69065 WP_000813258.1 NZ_CP018241:1812514-1812669 [Escherichia coli]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFVDYESEK

Nucleotide

• Length: 156 bp

>T69065 NZ_CP018241:1812514-1812669 [Escherichia coli]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTGCGAATCCGAACCGGTCAGACGGAGGTCGCTGTCTTCGTAGACTACGAATCTGAGAAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT69065 NZ_CP018241:c1812502-1812444 [Escherichia coli]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Toxin

• AlphaFold DB: A0A1Q4PF16

Antitoxin