Detailed information of TA system TA069060

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 1229540..1229765
• Replicon: chromosome
• Accession: NZ_CP018241
• Organism: Escherichia coli strain 319

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: -
• Locus tag: BFL24_RS06170
• Protein ID: WP_000813263.1
• Coordinates: 1229610..1229765 (+)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	1229540..1229598 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BFL24_RS06145	1224815..1225906	+	1092	WP_001205823.1	hypothetical protein	-
BFL24_RS06150	1225913..1226659	+	747	WP_000788751.1	ATP-binding protein	-
BFL24_RS06155	1226681..1227451	+	771	WP_000450992.1	DUF1627 domain-containing protein	-
BFL24_RS06160	1227467..1227880	+	414	WP_001151233.1	DUF977 family protein	-
BFL24_RS06165	1228232..1229005	-	774	WP_000160654.1	alpha/beta hydrolase	-
-	1229540..1229598	-	59	-	-	Antitoxin
BFL24_RS06170	1229610..1229765	+	156	WP_000813263.1	type I toxin-antitoxin system toxin HokD	Toxin
BFL24_RS06175	1229933..1230211	+	279	WP_001341388.1	hypothetical protein	-
BFL24_RS06180	1230213..1231262	+	1050	WP_001265172.1	DUF968 domain-containing protein	-
BFL24_RS06185	1231275..1231646	+	372	WP_001217436.1	RusA family crossover junction endodeoxyribonuclease	-
BFL24_RS06190	1231636..1232007	+	372	WP_000090264.1	antitermination protein	-
BFL24_RS06195	1232159..1232977	+	819	WP_000265265.1	CPBP family intramembrane metalloprotease	-
BFL24_RS06200	1233264..1233460	+	197	Protein_1105	TrmB family transcriptional regulator	-
BFL24_RS06205	1233598..1234311	+	714	WP_000261909.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5722.86 Da
• Isoelectric Point: 6.1531

>T69060 WP_000813263.1 NZ_CP018241:1229610-1229765 [Escherichia coli]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRVRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T69060 NZ_CP018241:1229610-1229765 [Escherichia coli]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAGTCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT69060 NZ_CP018241:c1229598-1229540 [Escherichia coli]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTAGATTTGCCGTTCATAGATTGAGCCTCA

Structures

Antitoxin