Detailed information of TA system TA069023

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2083197..2083422
• Replicon: chromosome
• Accession: NZ_CP018239
• Organism: Escherichia coli strain 272

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: A0A1Q4PF16
• Locus tag: BFL22_RS11340
• Protein ID: WP_000813258.1
• Coordinates: 2083197..2083352 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	2083364..2083422 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BFL22_RS11290	2078200..2078631	-	432	WP_001302123.1	tellurite resistance protein	-
BFL22_RS11305	2079082..2079795	-	714	WP_000301797.1	helix-turn-helix domain-containing protein	-
BFL22_RS31460	2079931..2080128	-	198	WP_000917763.1	hypothetical protein	-
BFL22_RS11315	2080353..2080907	-	555	WP_000640035.1	DUF1133 family protein	-
BFL22_RS11320	2080970..2081275	-	306	WP_001217444.1	RusA family crossover junction endodeoxyribonuclease	-
BFL22_RS11325	2081288..2082337	-	1050	WP_072643106.1	DUF968 domain-containing protein	-
BFL22_RS11330	2082339..2082611	-	273	WP_000191871.1	hypothetical protein	-
BFL22_RS11335	2082733..2083077	-	345	WP_000756596.1	YgiW/YdeI family stress tolerance OB fold protein	-
BFL22_RS11340	2083197..2083352	-	156	WP_000813258.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	2083364..2083422	+	59	-	-	Antitoxin
BFL22_RS11345	2083643..2084200	-	558	WP_000104474.1	DUF551 domain-containing protein	-
BFL22_RS11350	2084202..2084420	-	219	WP_000683609.1	DUF4014 family protein	-
BFL22_RS11355	2084548..2084859	-	312	WP_001289673.1	hypothetical protein	-
BFL22_RS11360	2084852..2085079	-	228	WP_000699809.1	hypothetical protein	-
BFL22_RS11365	2085076..2085357	-	282	WP_000603384.1	DNA-binding protein	-
BFL22_RS11370	2085390..2086106	-	717	WP_000450627.1	DUF1627 domain-containing protein	-
BFL22_RS31465	2086140..2086601	-	462	WP_000139447.1	replication protein	-
BFL22_RS31470	2086594..2087649	-	1056	WP_001356791.1	hypothetical protein	-
BFL22_RS11390	2087718..2088143	-	426	WP_000693878.1	Rha family transcriptional regulator	-
BFL22_RS11395	2088127..2088370	-	244	Protein_2011	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleG7'	2045750..2143910	98160

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5767.95 Da
• Isoelectric Point: 7.7942

>T69023 WP_000813258.1 NZ_CP018239:c2083352-2083197 [Escherichia coli]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFVDYESEK

Nucleotide

• Length: 156 bp

>T69023 NZ_CP018239:c2083352-2083197 [Escherichia coli]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTGCGAATCCGAACCGGTCAGACGGAGGTCGCTGTCTTCGTAGACTACGAATCTGAGAAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT69023 NZ_CP018239:2083364-2083422 [Escherichia coli]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Toxin

• AlphaFold DB: A0A1Q4PF16

Antitoxin