Detailed information of TA system TA069017

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 1179520..1179745
• Replicon: chromosome
• Accession: NZ_CP018239
• Organism: Escherichia coli strain 272

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: -
• Locus tag: BFL22_RS06000
• Protein ID: WP_000813263.1
• Coordinates: 1179590..1179745 (+)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	1179520..1179578 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BFL22_RS05975	1174795..1175886	+	1092	WP_001205823.1	hypothetical protein	-
BFL22_RS05980	1175893..1176639	+	747	WP_000788745.1	ATP-binding protein	-
BFL22_RS05985	1176661..1177431	+	771	WP_000450992.1	DUF1627 domain-containing protein	-
BFL22_RS05990	1177447..1177860	+	414	WP_001151233.1	DUF977 family protein	-
BFL22_RS05995	1178212..1178985	-	774	WP_000160650.1	alpha/beta hydrolase	-
-	1179520..1179578	-	59	-	-	Antitoxin
BFL22_RS06000	1179590..1179745	+	156	WP_000813263.1	type I toxin-antitoxin system toxin HokD	Toxin
BFL22_RS06005	1179913..1180191	+	279	WP_001341388.1	hypothetical protein	-
BFL22_RS06010	1180193..1181242	+	1050	WP_001265172.1	DUF968 domain-containing protein	-
BFL22_RS06015	1181255..1181626	+	372	WP_001217436.1	RusA family crossover junction endodeoxyribonuclease	-
BFL22_RS06020	1181616..1181987	+	372	WP_000090264.1	antitermination protein	-
BFL22_RS06025	1182139..1182957	+	819	WP_000265265.1	CPBP family intramembrane metalloprotease	-
BFL22_RS06030	1183244..1183440	+	197	Protein_1073	TrmB family transcriptional regulator	-
BFL22_RS06035	1183578..1184291	+	714	WP_000261909.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5722.86 Da
• Isoelectric Point: 6.1531

>T69017 WP_000813263.1 NZ_CP018239:1179590-1179745 [Escherichia coli]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRVRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T69017 NZ_CP018239:1179590-1179745 [Escherichia coli]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAGTCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT69017 NZ_CP018239:c1179578-1179520 [Escherichia coli]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTAGATTTGCCGTTCATAGATTGAGCCTCA

Structures

Antitoxin