Detailed information of TA system TA068970

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 292416..293110
• Replicon: chromosome
• Accession: NZ_CP018237
• Organism: Escherichia coli strain 155

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: C3TNX7
• Locus tag: BFL20_RS01440
• Protein ID: WP_001263495.1
• Coordinates: 292712..293110 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: BFL20_RS01435
• Protein ID: WP_000554757.1
• Coordinates: 292416..292709 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BFL20_RS31360	288127..288624	+	498	Protein_252	REP-associated tyrosine transposase RayT	-
BFL20_RS01420	288769..290481	-	1713	Protein_253	flagellar biosynthesis protein FlhA	-
BFL20_RS01425	290453..291238	+	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
BFL20_RS01430	291309..292364	+	1056	WP_001226186.1	DNA polymerase IV	-
BFL20_RS01435	292416..292709	+	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
BFL20_RS01440	292712..293110	+	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
BFL20_RS01445	293120..293572	+	453	WP_001059871.1	GNAT family N-acetyltransferase	-
BFL20_RS01450	293891..294094	+	204	Protein_259	RNA ligase RtcB family protein	-
BFL20_RS01455	294093..294614	+	522	Protein_260	peptide chain release factor H	-
BFL20_RS01460	294671..296128	-	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
BFL20_RS01465	296389..296847	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
-	297443..297523	+	81	NuclAT_12	-	-
-	297443..297523	+	81	NuclAT_12	-	-
-	297443..297523	+	81	NuclAT_12	-	-
-	297443..297523	+	81	NuclAT_12	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.86 Da
• Isoelectric Point: 8.0949

>T68970 WP_001263495.1 NZ_CP018237:292712-293110 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T68970 NZ_CP018237:292712-293110 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAGCTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACTTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAATCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT68970 WP_000554757.1 NZ_CP018237:292416-292709 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT68970 NZ_CP018237:292416-292709 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAATTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: S1FJN6