68941

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2846001..2846503 Replicon chromosome
Accession NZ_CP018206
Organism Escherichia coli strain MRSN346647

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag BSZ13_RS14855 Protein ID WP_000767829.1
Coordinates 2846001..2846255 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag BSZ13_RS14860 Protein ID WP_001259255.1
Coordinates 2846252..2846503 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BSZ13_RS14830 2841016..2841243 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
BSZ13_RS14835 2841257..2842315 - 1059 WP_000492315.1 transport protein YeeE -
BSZ13_RS14840 2842494..2843852 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
BSZ13_RS27700 2843842..2843904 - 63 WP_010723108.1 membrane protein YoeI -
BSZ13_RS14845 2844119..2845048 - 930 WP_000803351.1 LysR family transcriptional regulator -
BSZ13_RS14850 2845094..2845918 - 825 WP_000754749.1 SDR family oxidoreductase -
BSZ13_RS14855 2846001..2846255 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
BSZ13_RS14860 2846252..2846503 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
BSZ13_RS14865 2846786..2846836 + 51 WP_001364200.1 his operon leader peptide -
BSZ13_RS14875 2846982..2847881 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
BSZ13_RS14880 2847887..2849191 + 1305 WP_042031741.1 histidinol dehydrogenase -
BSZ13_RS14885 2849188..2850258 + 1071 WP_001682999.1 histidinol-phosphate transaminase -
BSZ13_RS14890 2850258..2851325 + 1068 WP_001563933.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T68941 WP_000767829.1 NZ_CP018206:c2846255-2846001 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T68941 NZ_CP018206:c2846255-2846001 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT68941 WP_001259255.1 NZ_CP018206:c2846503-2846252 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT68941 NZ_CP018206:c2846503-2846252 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LNR0

References