Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2846001..2846503 Replicon chromosome
Accession NZ_CP018206
Organism Escherichia coli strain MRSN346647

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag BSZ13_RS14855 Protein ID WP_000767829.1
Coordinates 2846001..2846255 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag BSZ13_RS14860 Protein ID WP_001259255.1
Coordinates 2846252..2846503 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BSZ13_RS14830 2841016..2841243 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
BSZ13_RS14835 2841257..2842315 - 1059 WP_000492315.1 transport protein YeeE -
BSZ13_RS14840 2842494..2843852 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
BSZ13_RS27700 2843842..2843904 - 63 WP_010723108.1 membrane protein YoeI -
BSZ13_RS14845 2844119..2845048 - 930 WP_000803351.1 LysR family transcriptional regulator -
BSZ13_RS14850 2845094..2845918 - 825 WP_000754749.1 SDR family oxidoreductase -
BSZ13_RS14855 2846001..2846255 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
BSZ13_RS14860 2846252..2846503 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
BSZ13_RS14865 2846786..2846836 + 51 WP_001364200.1 his operon leader peptide -
BSZ13_RS14875 2846982..2847881 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
BSZ13_RS14880 2847887..2849191 + 1305 WP_042031741.1 histidinol dehydrogenase -
BSZ13_RS14885 2849188..2850258 + 1071 WP_001682999.1 histidinol-phosphate transaminase -
BSZ13_RS14890 2850258..2851325 + 1068 WP_001563933.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T68941 WP_000767829.1 NZ_CP018206:c2846255-2846001 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T68941 NZ_CP018206:c2846255-2846001 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT68941 WP_001259255.1 NZ_CP018206:c2846503-2846252 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT68941 NZ_CP018206:c2846503-2846252 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References