Detailed information of TA system TA068926

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 2078466..2078837
• Replicon: chromosome
• Accession: NZ_CP018206
• Organism: Escherichia coli strain MRSN346647

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: F4VC37
• Locus tag: BSZ13_RS10640
• Protein ID: WP_001317028.1
• Coordinates: 2078643..2078837 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	2078466..2078644 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BSZ13_RS10615	2074421..2075794	+	1374	WP_001551034.1	ATP-dependent RNA helicase DbpA	-
BSZ13_RS10620	2075923..2076858	-	936	WP_001157407.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
BSZ13_RS10625	2076910..2078145	-	1236	WP_072617251.1	site-specific integrase	-
BSZ13_RS10630	2078147..2078362	-	216	WP_000079604.1	excisionase XisR	-
BSZ13_RS10635	2078441..2078650	-	210	WP_000276809.1	double-strand break reduction protein RcbA	-
-	2078466..2078644	+	179	NuclAT_0	-	Antitoxin
-	2078466..2078644	+	179	NuclAT_0	-	Antitoxin
-	2078466..2078644	+	179	NuclAT_0	-	Antitoxin
-	2078466..2078644	+	179	NuclAT_0	-	Antitoxin
BSZ13_RS10640	2078643..2078837	-	195	WP_001317028.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
BSZ13_RS10645	2078893..2079702	-	810	WP_000166319.1	recombination protein RecT	-
BSZ13_RS10650	2079695..2082295	-	2601	WP_072617252.1	exodeoxyribonuclease VIII	-
BSZ13_RS10655	2082395..2082670	-	276	WP_001372690.1	hypothetical protein	-
BSZ13_RS10660	2082745..2082915	-	171	WP_065336296.1	hypothetical protein	-
BSZ13_RS10665	2082915..2083136	-	222	WP_000560223.1	killing protein KilR	-
BSZ13_RS10670	2083558..2083710	-	153	WP_000379561.1	DUF1391 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2076910..2122875	45965

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7018.89 Da
• Isoelectric Point: 8.9538

>T68926 WP_001317028.1 NZ_CP018206:c2078837-2078643 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T68926 NZ_CP018206:c2078837-2078643 [Escherichia coli]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin

Nucleotide

• Length: 179 bp

>AT68926 NZ_CP018206:2078466-2078644 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A7L6LHV7

Antitoxin