Detailed information of TA system TA068920

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 837405..838099
• Replicon: chromosome
• Accession: NZ_CP018206
• Organism: Escherichia coli strain MRSN346647

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q0T7Q5
• Locus tag: BSZ13_RS04220
• Protein ID: WP_001263491.1
• Coordinates: 837701..838099 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: L4JHF0
• Locus tag: BSZ13_RS04215
• Protein ID: WP_000554755.1
• Coordinates: 837405..837698 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BSZ13_RS04190	833026..833382	-	357	WP_001030484.1	type II toxin-antitoxin system HicB family antitoxin	-
BSZ13_RS04195	833375..833653	-	279	WP_000598760.1	type II toxin-antitoxin system HicA family toxin	-
BSZ13_RS04200	833758..835470	-	1713	Protein_741	flagellar biosynthesis protein FlhA	-
BSZ13_RS04205	835442..836227	+	786	WP_019842510.1	putative lateral flagellar export/assembly protein LafU	-
BSZ13_RS04210	836298..837353	+	1056	WP_001550655.1	DNA polymerase IV	-
BSZ13_RS04215	837405..837698	+	294	WP_000554755.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
BSZ13_RS04220	837701..838099	+	399	WP_001263491.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
BSZ13_RS04225	838109..838561	+	453	WP_019842500.1	GNAT family N-acetyltransferase	-
BSZ13_RS04230	838807..839013	+	207	Protein_747	RtcB family protein	-
BSZ13_RS04235	839009..839361	+	353	Protein_748	peptide chain release factor H	-
BSZ13_RS04240	839418..840875	-	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
BSZ13_RS04245	841136..841594	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
BSZ13_RS04250	841686..842930	+	1245	WP_000189541.1	esterase FrsA	-
-	842190..842270	+	81	NuclAT_11	-	-
-	842190..842270	+	81	NuclAT_11	-	-
-	842190..842270	+	81	NuclAT_11	-	-
-	842190..842270	+	81	NuclAT_11	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15457.86 Da
• Isoelectric Point: 8.0949

>T68920 WP_001263491.1 NZ_CP018206:837701-838099 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T68920 NZ_CP018206:837701-838099 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAGCCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGAAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11235.78 Da
• Isoelectric Point: 5.2170

>AT68920 WP_000554755.1 NZ_CP018206:837405-837698 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT68920 NZ_CP018206:837405-837698 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAATCCAGCAAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CAGAACAAGAGGAGAAAAAACTGGTGAAGGCGCGCTTCCGTCCGAGTGCCGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: A0A4P7TPK7

Antitoxin

• AlphaFold DB: A0A829G9H5