Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 29608..30201 Replicon chromosome
Accession NZ_CP018202
Organism Pseudomonas syringae pv. actinidiae ICMP 9853

Toxin (Protein)


Gene name graT Uniprot ID A0A2G7PD43
Locus tag JN853_RS00175 Protein ID WP_003377943.1
Coordinates 29923..30201 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name graA Uniprot ID Q88B19
Locus tag JN853_RS00170 Protein ID WP_005739893.1
Coordinates 29608..29913 (-) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JN853_RS00130 25297..25989 + 693 WP_003377932.1 HAD family hydrolase -
JN853_RS00135 26064..26396 - 333 WP_003377934.1 hypothetical protein -
JN853_RS00140 26495..26920 - 426 WP_003377936.1 secondary thiamine-phosphate synthase enzyme YjbQ -
JN853_RS00150 27163..28500 - 1338 WP_003377937.1 ammonium transporter -
JN853_RS00155 28535..28873 - 339 WP_002555808.1 P-II family nitrogen regulator -
JN853_RS00165 29293..29574 + 282 WP_003377939.1 accessory factor UbiK family protein -
JN853_RS00170 29608..29913 - 306 WP_005739893.1 HigA family addiction module antidote protein Antitoxin
JN853_RS00175 29923..30201 - 279 WP_003377943.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
JN853_RS31565 30314..31893 + 1580 WP_085991147.1 IS3-like element ISPsy31 family transposase -
JN853_RS00190 31931..32404 - 474 Protein_33 helix-turn-helix domain-containing protein -
JN853_RS00195 32401..32949 - 549 WP_003377947.1 heteromeric transposase endonuclease subunit TnsA -
JN853_RS00200 33205..33855 + 651 Protein_35 ATP-binding protein -
JN853_RS00205 33892..34824 + 933 WP_003377951.1 type III effector -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(5-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(6-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10512.18 Da        Isoelectric Point: 9.1490

>T68889 WP_003377943.1 NZ_CP018202:c30201-29923 [Pseudomonas syringae pv. actinidiae ICMP 9853]
MIVSFKCVHTRYLFLQGKTRLWPSIKSVAERKLAMLDAATSILDLRSPPGNRLEVLDGSRSGQYSVRINAQFRICFVWSI
NGPEDVEIVDYH

Download         Length: 279 bp

>T68889 NZ_CP018202:c30201-29923 [Pseudomonas syringae pv. actinidiae ICMP 9853]
ATGATTGTGAGTTTTAAGTGCGTGCACACGAGATATCTCTTTTTACAAGGCAAGACGCGGTTGTGGCCATCGATTAAATC
TGTTGCCGAGCGCAAGCTCGCAATGCTTGATGCTGCTACCTCGATTTTGGATCTGCGCTCTCCTCCCGGCAATCGACTAG
AGGTGCTTGATGGAAGTCGTTCTGGCCAATACAGCGTACGTATAAACGCTCAATTCAGGATTTGCTTCGTTTGGAGCATC
AACGGTCCCGAGGATGTAGAAATTGTCGATTATCACTGA

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11158.01 Da        Isoelectric Point: 7.7509

>AT68889 WP_005739893.1 NZ_CP018202:c29913-29608 [Pseudomonas syringae pv. actinidiae ICMP 9853]
MNKNGMRPVHPGEVLKEEYLEPMGLTAAALARALKVSTPTVNDIVLQRRGVSADVALRLAVCLETSPEFWLNLQLAYDLR
KAETEKGAQIREQVKCLAHCA

Download         Length: 306 bp

>AT68889 NZ_CP018202:c29913-29608 [Pseudomonas syringae pv. actinidiae ICMP 9853]
ATGAACAAAAACGGAATGCGCCCCGTCCATCCAGGCGAGGTTCTGAAAGAGGAATATCTTGAGCCGATGGGGCTGACCGC
CGCAGCGTTGGCCAGAGCCCTTAAGGTTTCTACGCCGACGGTGAACGACATCGTGCTCCAGCGCAGAGGTGTGAGCGCTG
ATGTAGCGCTGCGTCTGGCTGTATGCCTGGAGACGAGTCCTGAGTTCTGGCTTAACCTGCAGCTTGCCTACGATCTGCGC
AAGGCAGAGACAGAGAAGGGCGCGCAGATTCGTGAGCAGGTCAAATGTCTGGCCCACTGCGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2G7PD43

Antitoxin

Source ID Structure
AlphaFold DB A0A0K8LYY6

References