Detailed information of TA system TA068880

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-couple_hipB
• Location: 1446458..1447115
• Replicon: chromosome
• Accession: NZ_CP018189
• Organism: Streptococcus salivarius strain ICDC3

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A5C1JKZ1
• Locus tag: BSR20_RS06495
• Protein ID: WP_045001823.1
• Coordinates: 1446762..1447115 (-)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: BSR20_RS06490
• Protein ID: WP_014633320.1
• Coordinates: 1446458..1446760 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BSR20_RS06480	1442639..1443292	-	654	WP_013990814.1	hypothetical protein	-
BSR20_RS06485	1443304..1446294	-	2991	WP_156209804.1	type I restriction endonuclease subunit R	-
BSR20_RS06490	1446458..1446760	-	303	WP_014633320.1	helix-turn-helix transcriptional regulator	Antitoxin
BSR20_RS06495	1446762..1447115	-	354	WP_045001823.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BSR20_RS06500	1447352..1448116	-	765	WP_156209806.1	SDR family NAD(P)-dependent oxidoreductase	-
BSR20_RS06505	1448148..1449305	-	1158	WP_156209807.1	DUF2974 domain-containing protein	-
BSR20_RS06510	1449415..1450041	+	627	WP_045771826.1	Pr6Pr family membrane protein	-
BSR20_RS06515	1450079..1450921	-	843	WP_070578032.1	Cof-type HAD-IIB family hydrolase	-
BSR20_RS06520	1451073..1451462	+	390	WP_155213994.1	sodium transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	erm(B)	psaA	1343679..1465950	122271

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (35-109)

Antitoxin

• Cro/C1-type helix-turn-helix domain (39-91)

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 14056.43 Da
• Isoelectric Point: 10.3788

>T68880 WP_045001823.1 NZ_CP018189:c1447115-1446762 [Streptococcus salivarius]
MYPIHFYKDRNGHEPVREYIETLSINSSKDSRIKLHKIQDYLTMLKNRGTMIGEPFIKHLEGEIWELRPLRDRILFAAWL
DDGFILLHHFVKKTQKTPRRELMKAQKALDDIRKRGL

Nucleotide

• Length: 354 bp

>T68880 NZ_CP018189:c1447115-1446762 [Streptococcus salivarius]
ATGTATCCTATTCATTTTTATAAAGATAGAAATGGACATGAACCTGTTAGAGAGTATATTGAAACATTATCCATAAATTC
AAGCAAAGATTCTAGAATCAAATTACATAAAATTCAAGACTACCTGACTATGTTAAAAAATAGAGGAACCATGATTGGTG
AACCATTCATTAAACATTTGGAAGGAGAGATTTGGGAACTAAGACCCTTAAGAGATAGAATTTTATTTGCAGCATGGCTA
GATGATGGTTTTATTTTGCTTCATCATTTTGTGAAAAAGACACAAAAGACTCCAAGACGTGAGCTGATGAAGGCTCAAAA
AGCTTTAGATGATATTAGAAAGCGAGGATTGTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 10965.90 Da
• Isoelectric Point: 10.0976

>AT68880 WP_014633320.1 NZ_CP018189:c1446760-1446458 [Streptococcus salivarius]
MAREKNPAIGSSWDDLQKTIFTKEEIAASELRVALMLEIIDARKKQGISQKKLEELSGVKQPVIARMETGKTAPQLDTVL
KILASLGKTLAVVPLDSHVK

Nucleotide

• Length: 303 bp

>AT68880 NZ_CP018189:c1446760-1446458 [Streptococcus salivarius]
ATGGCTCGAGAAAAGAATCCAGCAATTGGAAGTAGTTGGGATGATTTACAGAAAACTATTTTCACTAAAGAGGAAATCGC
GGCAAGTGAACTCCGTGTCGCACTGATGCTTGAGATTATTGATGCTCGTAAAAAACAAGGCATTTCTCAAAAGAAACTTG
AAGAGCTAAGTGGTGTCAAACAACCTGTTATCGCACGTATGGAGACAGGTAAAACAGCACCTCAGTTAGATACGGTTCTT
AAAATACTGGCGAGTCTAGGTAAGACATTGGCGGTAGTCCCACTTGATTCACATGTGAAGTAA

Structures

Toxin

• AlphaFold DB: A0A5C1JKZ1