Detailed information of TA system TA068874

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-couple_hipB
• Location: 1446434..1447091
• Replicon: chromosome
• Accession: NZ_CP018186
• Organism: Streptococcus salivarius strain ICDC1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A5C1JKZ1
• Locus tag: BSR14_RS06495
• Protein ID: WP_045001823.1
• Coordinates: 1446738..1447091 (-)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: BSR14_RS06490
• Protein ID: WP_014633320.1
• Coordinates: 1446434..1446736 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BSR14_RS06480	1442615..1443268	-	654	WP_013990814.1	hypothetical protein	-
BSR14_RS06485	1443280..1446270	-	2991	WP_156209804.1	type I restriction endonuclease subunit R	-
BSR14_RS06490	1446434..1446736	-	303	WP_014633320.1	helix-turn-helix transcriptional regulator	Antitoxin
BSR14_RS06495	1446738..1447091	-	354	WP_045001823.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BSR14_RS06500	1447328..1448092	-	765	WP_156209806.1	SDR family NAD(P)-dependent oxidoreductase	-
BSR14_RS06505	1448124..1449281	-	1158	WP_156209807.1	DUF2974 domain-containing protein	-
BSR14_RS06510	1449391..1450017	+	627	WP_045771826.1	Pr6Pr family membrane protein	-
BSR14_RS06515	1450055..1450897	-	843	WP_070578032.1	Cof-type HAD-IIB family hydrolase	-
BSR14_RS06520	1451049..1451438	+	390	WP_155213994.1	sodium transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	erm(B)	psaA	1343655..1465926	122271

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (35-109)

Antitoxin

• Cro/C1-type helix-turn-helix domain (39-91)

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 14056.43 Da
• Isoelectric Point: 10.3788

>T68874 WP_045001823.1 NZ_CP018186:c1447091-1446738 [Streptococcus salivarius]
MYPIHFYKDRNGHEPVREYIETLSINSSKDSRIKLHKIQDYLTMLKNRGTMIGEPFIKHLEGEIWELRPLRDRILFAAWL
DDGFILLHHFVKKTQKTPRRELMKAQKALDDIRKRGL

Nucleotide

• Length: 354 bp

>T68874 NZ_CP018186:c1447091-1446738 [Streptococcus salivarius]
ATGTATCCTATTCATTTTTATAAAGATAGAAATGGACATGAACCTGTTAGAGAGTATATTGAAACATTATCCATAAATTC
AAGCAAAGATTCTAGAATCAAATTACATAAAATTCAAGACTACCTGACTATGTTAAAAAATAGAGGAACCATGATTGGTG
AACCATTCATTAAACATTTGGAAGGAGAGATTTGGGAACTAAGACCCTTAAGAGATAGAATTTTATTTGCAGCATGGCTA
GATGATGGTTTTATTTTGCTTCATCATTTTGTGAAAAAGACACAAAAGACTCCAAGACGTGAGCTGATGAAGGCTCAAAA
AGCTTTAGATGATATTAGAAAGCGAGGATTGTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 10965.90 Da
• Isoelectric Point: 10.0976

>AT68874 WP_014633320.1 NZ_CP018186:c1446736-1446434 [Streptococcus salivarius]
MAREKNPAIGSSWDDLQKTIFTKEEIAASELRVALMLEIIDARKKQGISQKKLEELSGVKQPVIARMETGKTAPQLDTVL
KILASLGKTLAVVPLDSHVK

Nucleotide

• Length: 303 bp

>AT68874 NZ_CP018186:c1446736-1446434 [Streptococcus salivarius]
ATGGCTCGAGAAAAGAATCCAGCAATTGGAAGTAGTTGGGATGATTTACAGAAAACTATTTTCACTAAAGAGGAAATCGC
GGCAAGTGAACTCCGTGTCGCACTGATGCTTGAGATTATTGATGCTCGTAAAAAACAAGGCATTTCTCAAAAGAAACTTG
AAGAGCTAAGTGGTGTCAAACAACCTGTTATCGCACGTATGGAGACAGGTAAAACAGCACCTCAGTTAGATACGGTTCTT
AAAATACTGGCGAGTCTAGGTAAGACATTGGCGGTAGTCCCACTTGATTCACATGTGAAGTAA

Structures

Toxin

• AlphaFold DB: A0A5C1JKZ1