68808

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2235795..2236297 Replicon chromosome
Accession NZ_CP018121
Organism Escherichia coli strain MRSN346355

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag BSG24_RS11760 Protein ID WP_000767829.1
Coordinates 2235795..2236049 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag BSG24_RS11765 Protein ID WP_001259255.1
Coordinates 2236046..2236297 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BSG24_RS11735 2230810..2231037 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
BSG24_RS11740 2231051..2232109 - 1059 WP_000492333.1 transport protein YeeE -
BSG24_RS11745 2232288..2233646 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
BSG24_RS28180 2233636..2233698 - 63 WP_010723108.1 membrane protein YoeI -
BSG24_RS11750 2233913..2234842 - 930 WP_000803351.1 LysR family transcriptional regulator -
BSG24_RS11755 2234888..2235712 - 825 WP_000754737.1 SDR family oxidoreductase -
BSG24_RS11760 2235795..2236049 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
BSG24_RS11765 2236046..2236297 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
BSG24_RS11770 2236580..2236630 + 51 WP_001364200.1 his operon leader peptide -
BSG24_RS11780 2236776..2237675 + 900 WP_000131792.1 ATP phosphoribosyltransferase -
BSG24_RS11785 2237681..2238985 + 1305 WP_001367667.1 histidinol dehydrogenase -
BSG24_RS11790 2238982..2240052 + 1071 WP_000108941.1 histidinol-phosphate transaminase -
BSG24_RS11795 2240052..2241119 + 1068 WP_000080141.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T68808 WP_000767829.1 NZ_CP018121:c2236049-2235795 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T68808 NZ_CP018121:c2236049-2235795 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT68808 WP_001259255.1 NZ_CP018121:c2236297-2236046 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT68808 NZ_CP018121:c2236297-2236046 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LNR0

References