Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type I Classification (family/domain) hok-sok/-
Location 73450..73714 Replicon plasmid pMRSN346638_119.3
Accession NZ_CP018116
Organism Escherichia coli strain MRSN346638

Toxin (Protein)


Gene name pndA Uniprot ID E6BRV3
Locus tag BSG23_RS25470 Protein ID WP_001303307.1
Coordinates 73562..73714 (+) Length 51 a.a.

Antitoxin (RNA)


Gene name pndB
Locus tag -
Coordinates 73450..73512 (-)

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BSG23_RS25455 68689..70980 - 2292 WP_001289271.1 hypothetical protein -
BSG23_RS25460 70973..72043 - 1071 WP_000151575.1 IncI1-type conjugal transfer protein TrbB -
BSG23_RS25465 72062..73270 - 1209 WP_000121273.1 IncI1-type conjugal transfer protein TrbA -
- 73450..73512 - 63 NuclAT_0 - Antitoxin
- 73450..73512 - 63 NuclAT_0 - Antitoxin
- 73450..73512 - 63 NuclAT_0 - Antitoxin
- 73450..73512 - 63 NuclAT_0 - Antitoxin
BSG23_RS25470 73562..73714 + 153 WP_001303307.1 Hok/Gef family protein Toxin
BSG23_RS25475 73786..74037 - 252 WP_001291965.1 hypothetical protein -
- 74424..74475 - 52 NuclAT_1 - -
- 74424..74475 - 52 NuclAT_1 - -
- 74424..74475 - 52 NuclAT_1 - -
- 74424..74475 - 52 NuclAT_1 - -
BSG23_RS28730 74961..75137 - 177 WP_001054900.1 hypothetical protein -
BSG23_RS25495 75529..75738 + 210 WP_000062603.1 HEAT repeat domain-containing protein -
BSG23_RS25500 75810..76472 - 663 WP_000644794.1 plasmid IncI1-type surface exclusion protein ExcA -
BSG23_RS25505 76543..78711 - 2169 WP_071940810.1 DotA/TraY family protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaTEM-1B / tet(A) / erm(42) / floR - 1..119254 119254


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(7-46)


Sequences


Toxin        


Download         Length: 51 a.a.        Molecular weight: 5817.21 Da        Isoelectric Point: 8.7948

>T68786 WP_001303307.1 NZ_CP018116:73562-73714 [Escherichia coli]
MPQRTFLMMLIVICVTILCFVWMVRDSLCVLRLQQGNTVLVATLAYEVKR

Download         Length: 153 bp

>T68786 NZ_CP018116:73562-73714 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCATCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGTACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin


Download         Length: 63 bp

>AT68786 NZ_CP018116:c73512-73450 [Escherichia coli]
TCGAAGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A634Q5L1


Antitoxin

Download structure file

References