Detailed information of TA system TA068779

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mqsRA (relBE)/MqsR-MqsA
• Location: 3336084..3336777
• Replicon: chromosome
• Accession: NZ_CP018115
• Organism: Escherichia coli strain MRSN346638

Toxin (Protein)

• Gene name: mqsR
• Uniprot ID: S1EZG2
• Locus tag: BSG23_RS17205
• Protein ID: WP_000415584.1
• Coordinates: 3336481..3336777 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: mqsA
• Uniprot ID: S1EBV2
• Locus tag: BSG23_RS17200
• Protein ID: WP_000650107.1
• Coordinates: 3336084..3336479 (-)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BSG23_RS17190	3333272..3334879	-	1608	WP_001295629.1	ABC transporter substrate-binding protein	-
BSG23_RS17195	3335017..3335988	-	972	WP_170386723.1	IS30-like element ISApl1 family transposase	-
BSG23_RS17200	3336084..3336479	-	396	WP_000650107.1	type II toxin-antitoxin system antitoxin MqsA	Antitoxin
BSG23_RS17205	3336481..3336777	-	297	WP_000415584.1	type II toxin-antitoxin system toxin MqsR	Toxin
BSG23_RS17210	3336982..3337464	-	483	WP_000183505.1	transcriptional regulator	-
BSG23_RS17215	3337517..3337909	-	393	WP_000712658.1	OB fold stress tolerance protein YgiW	-
BSG23_RS17220	3338061..3338720	+	660	WP_001221493.1	two-component system response regulator QseB	-
BSG23_RS17225	3338717..3340066	+	1350	WP_000673402.1	two-component system sensor histidine kinase QseC	-
BSG23_RS17230	3340112..3340444	-	333	WP_023149630.1	DUF2645 family protein	-
BSG23_RS17235	3340763..3341344	+	582	WP_000065430.1	NADPH:quinone oxidoreductase MdaB	-
BSG23_RS17240	3341375..3341689	+	315	WP_000958598.1	antibiotic biosynthesis monooxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3335017..3335940	923

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin MqsR (5-97)

Antitoxin

• Zinc finger, MqsA-type (3-47)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11231.96 Da
• Isoelectric Point: 8.9070

>T68779 WP_000415584.1 NZ_CP018115:c3336777-3336481 [Escherichia coli]
MEKRTPHTRLSQVKKLVNAGQVRTTRSALLNADELGLDFDGMCNVIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQV
YLKITVIHDVLIVSFKEK

Nucleotide

• Length: 297 bp

>T68779 NZ_CP018115:c3336777-3336481 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14703.10 Da
• Isoelectric Point: 9.2136

>AT68779 WP_000650107.1 NZ_CP018115:c3336479-3336084 [Escherichia coli]
MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESIMNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKK
LSLTQKEASEIFGGGVNAFSRYEKGNAQPHPSTIKLLRVLDKHPELLNEIR

Nucleotide

• Length: 396 bp

>AT68779 NZ_CP018115:c3336479-3336084 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Structures

Toxin

• PDB: 3HI2

Antitoxin

• AlphaFold DB: A0A7U9LU65