Detailed information of TA system TA068776

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2995347..2996074
• Replicon: chromosome
• Accession: NZ_CP018115
• Organism: Escherichia coli strain MRSN346638

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A829L2T9
• Locus tag: BSG23_RS15460
• Protein ID: WP_001521139.1
• Coordinates: 2995763..2996074 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: BSG23_RS15455
• Protein ID: WP_000126294.1
• Coordinates: 2995347..2995766 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BSG23_RS15435	2990432..2990959	-	528	WP_001078777.1	electron transport protein HydN	-
BSG23_RS15440	2991108..2992118	-	1011	WP_015912547.1	DNA-binding transcriptional regulator AscG	-
BSG23_RS15445	2992378..2993835	+	1458	WP_020234027.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
BSG23_RS15450	2993844..2995268	+	1425	WP_020234028.1	6-phospho-beta-glucosidase AscB	-
BSG23_RS15455	2995347..2995766	-	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
BSG23_RS15460	2995763..2996074	-	312	WP_001521139.1	type II toxin-antitoxin system HigB family toxin	Toxin
BSG23_RS15465	2996241..2996711	-	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
BSG23_RS15470	2996704..2997114	-	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
BSG23_RS15475	2997111..2997878	-	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
BSG23_RS15480	2997878..2998420	-	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
BSG23_RS15485	2998430..3000139	-	1710	WP_001521140.1	formate hydrogenlyase subunit HycE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12493.23 Da
• Isoelectric Point: 9.4783

>T68776 WP_001521139.1 NZ_CP018115:c2996074-2995763 [Escherichia coli]
MHIISKAPFEECARKYPNDALALYSLYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T68776 NZ_CP018115:c2996074-2995763 [Escherichia coli]
ATGCACATCATTTCGAAAGCGCCATTTGAAGAGTGTGCGCGCAAATATCCTAACGACGCTCTGGCTCTATACTCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAGATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT68776 WP_000126294.1 NZ_CP018115:c2995766-2995347 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT68776 NZ_CP018115:c2995766-2995347 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGTAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAATATGAAAACAACAACGAAAAGTTTGCCGAGTTCGACAAAGCCGTTGCAGCAATGCCGGTT
GGCGTGGCGTTACTTCGTACGCTGATTGATCAACATAATCTGACCTACGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGGCAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGTGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A829L2T9