Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 280740..281281 Replicon chromosome
Accession NZ_CP018115
Organism Escherichia coli strain MRSN346638

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag BSG23_RS01330 Protein ID WP_000615983.1
Coordinates 280740..281018 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag BSG23_RS01335 Protein ID WP_000729703.1
Coordinates 281021..281281 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BSG23_RS01315 278322..278900 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
BSG23_RS01320 279106..279873 + 768 WP_000333380.1 class II glutamine amidotransferase -
BSG23_RS01325 279844..280584 - 741 WP_001225679.1 murein L,D-transpeptidase -
BSG23_RS01330 280740..281018 - 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
BSG23_RS01335 281021..281281 - 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
BSG23_RS01340 281338..282558 - 1221 WP_000343760.1 ISL3-like element ISKox3 family transposase -
BSG23_RS01345 282815..283564 + 750 WP_000056849.1 C40 family peptidase -
BSG23_RS01355 283740..284237 + 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
BSG23_RS01365 284461..286173 - 1713 Protein_248 flagellar biosynthesis protein FlhA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 273021..307090 34069
- flank IS/Tn - - 281338..282558 1220


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T68761 WP_000615983.1 NZ_CP018115:c281018-280740 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T68761 NZ_CP018115:c281018-280740 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATATTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT68761 WP_000729703.1 NZ_CP018115:c281281-281021 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT68761 NZ_CP018115:c281281-281021 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCAGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCATTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References