Detailed information of TA system TA068753

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 74522..74786
• Replicon: plasmid pMRSN346595_120.3
• Accession: NZ_CP018110
• Organism: Escherichia coli strain MRSN346595

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: E6BRV3
• Locus tag: BSG21_RS25455
• Protein ID: WP_001303307.1
• Coordinates: 74634..74786 (+)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	74522..74584 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BSG21_RS25440	69761..72052	-	2292	WP_001289271.1	hypothetical protein	-
BSG21_RS25445	72045..73115	-	1071	WP_000151575.1	IncI1-type conjugal transfer protein TrbB	-
BSG21_RS25450	73134..74342	-	1209	WP_000121273.1	IncI1-type conjugal transfer protein TrbA	-
-	74522..74584	-	63	NuclAT_0	-	Antitoxin
-	74522..74584	-	63	NuclAT_0	-	Antitoxin
-	74522..74584	-	63	NuclAT_0	-	Antitoxin
-	74522..74584	-	63	NuclAT_0	-	Antitoxin
BSG21_RS25455	74634..74786	+	153	WP_001303307.1	Hok/Gef family protein	Toxin
BSG21_RS25460	74858..75109	-	252	WP_001291965.1	hypothetical protein	-
-	75496..75547	-	52	NuclAT_1	-	-
-	75496..75547	-	52	NuclAT_1	-	-
-	75496..75547	-	52	NuclAT_1	-	-
-	75496..75547	-	52	NuclAT_1	-	-
BSG21_RS28810	76033..76209	-	177	WP_001054900.1	hypothetical protein	-
BSG21_RS25480	76601..76810	+	210	WP_000062603.1	HEAT repeat domain-containing protein	-
BSG21_RS25485	76882..77544	-	663	WP_000644794.1	plasmid IncI1-type surface exclusion protein ExcA	-
BSG21_RS25490	77615..79783	-	2169	WP_071940810.1	DotA/TraY family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B / tet(A) / erm(42) / floR	-	1..120326	120326

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5817.21 Da
• Isoelectric Point: 8.7948

>T68753 WP_001303307.1 NZ_CP018110:74634-74786 [Escherichia coli]
MPQRTFLMMLIVICVTILCFVWMVRDSLCVLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T68753 NZ_CP018110:74634-74786 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCATCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGTACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin

Nucleotide

• Length: 63 bp

>AT68753 NZ_CP018110:c74584-74522 [Escherichia coli]
TCGAAGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: A0A634Q5L1

Antitoxin