Detailed information of TA system TA068747

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 3441648..3442447
• Replicon: chromosome
• Accession: NZ_CP018109
• Organism: Escherichia coli strain MRSN346595

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: D3GWU5
• Locus tag: BSG21_RS17750
• Protein ID: WP_000347272.1
• Coordinates: 3441983..3442447 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: S1EB98
• Locus tag: BSG21_RS17745
• Protein ID: WP_001307405.1
• Coordinates: 3441648..3441983 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BSG21_RS17730	3437433..3438203	-	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-
BSG21_RS17735	3438219..3439553	-	1335	WP_000599651.1	galactarate/glucarate/glycerate transporter GarP	-
BSG21_RS17740	3439928..3441499	+	1572	WP_001273738.1	galactarate dehydratase	-
BSG21_RS17745	3441648..3441983	+	336	WP_001307405.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
BSG21_RS17750	3441983..3442447	+	465	WP_000347272.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
BSG21_RS17755	3442502..3443311	-	810	WP_000072171.1	aga operon transcriptional regulator AgaR	-
BSG21_RS17765	3443560..3444840	+	1281	WP_001521382.1	tagatose-bisphosphate aldolase subunit KbaZ	-
BSG21_RS17770	3444863..3445336	+	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
BSG21_RS17775	3445347..3446126	+	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
BSG21_RS17780	3446116..3446994	+	879	WP_001298314.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
BSG21_RS17785	3447012..3447446	+	435	WP_000948824.1	PTS sugar transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3432723..3442447	9724

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17832.26 Da
• Isoelectric Point: 9.6924

>T68747 WP_000347272.1 NZ_CP018109:3441983-3442447 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEEPH

Nucleotide

• Length: 465 bp

>T68747 NZ_CP018109:3441983-3442447 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGACAGAAGAACCCCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12358.94 Da
• Isoelectric Point: 4.8616

>AT68747 WP_001307405.1 NZ_CP018109:3441648-3441983 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT68747 NZ_CP018109:3441648-3441983 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: A0A829KUD2

Antitoxin

• AlphaFold DB: A0A0E0XVC7