Detailed information of TA system TA068729

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 288108..288802
• Replicon: chromosome
• Accession: NZ_CP018109
• Organism: Escherichia coli strain MRSN346595

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q47157
• Locus tag: BSG21_RS01385
• Protein ID: WP_001263489.1
• Coordinates: 288404..288802 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: BSG21_RS01380
• Protein ID: WP_000554758.1
• Coordinates: 288108..288401 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BSG21_RS01355	283740..284237	+	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-
BSG21_RS01365	284461..286173	-	1713	Protein_248	flagellar biosynthesis protein FlhA	-
BSG21_RS01370	286145..286930	+	786	WP_000207552.1	putative lateral flagellar export/assembly protein LafU	-
BSG21_RS01375	287001..288056	+	1056	WP_001226164.1	DNA polymerase IV	-
BSG21_RS01380	288108..288401	+	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
BSG21_RS01385	288404..288802	+	399	WP_001263489.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
BSG21_RS01390	288812..289264	+	453	WP_001059892.1	GNAT family N-acetyltransferase	-
BSG21_RS01400	289582..289788	+	207	Protein_254	RtcB family protein	-
BSG21_RS01405	289784..290305	+	522	Protein_255	peptide chain release factor H	-
BSG21_RS01410	290362..291819	-	1458	WP_001292994.1	cytosol nonspecific dipeptidase	-
BSG21_RS01415	292080..292538	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
-	293134..293214	+	81	NuclAT_11	-	-
-	293134..293214	+	81	NuclAT_11	-	-
-	293134..293214	+	81	NuclAT_11	-	-
-	293134..293214	+	81	NuclAT_11	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	gmhA/lpcA	273021..307090	34069

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15487.84 Da
• Isoelectric Point: 7.4215

>T68729 WP_001263489.1 NZ_CP018109:288404-288802 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T68729 NZ_CP018109:288404-288802 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT68729 WP_000554758.1 NZ_CP018109:288108-288401 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT68729 NZ_CP018109:288108-288401 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: A0A090J8B1

Antitoxin

• AlphaFold DB: S1QAE3