Detailed information of TA system TA068720

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 162156..162420
• Replicon: plasmid pMR0716_tem1
• Accession: NZ_CP018104
• Organism: Escherichia coli strain MRSN352231

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: E6BRV3
• Locus tag: BSG22_RS26120
• Protein ID: WP_001303307.1
• Coordinates: 162268..162420 (+)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	162156..162218 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BSG22_RS26105	157395..159686	-	2292	WP_001289271.1	hypothetical protein	-
BSG22_RS26110	159679..160749	-	1071	WP_000151575.1	IncI1-type conjugal transfer protein TrbB	-
BSG22_RS26115	160768..161976	-	1209	WP_000121273.1	IncI1-type conjugal transfer protein TrbA	-
-	162156..162218	-	63	NuclAT_0	-	Antitoxin
-	162156..162218	-	63	NuclAT_0	-	Antitoxin
-	162156..162218	-	63	NuclAT_0	-	Antitoxin
-	162156..162218	-	63	NuclAT_0	-	Antitoxin
BSG22_RS26120	162268..162420	+	153	WP_001303307.1	Hok/Gef family protein	Toxin
BSG22_RS26125	162492..162743	-	252	WP_001291965.1	hypothetical protein	-
-	163130..163181	-	52	NuclAT_1	-	-
-	163130..163181	-	52	NuclAT_1	-	-
-	163130..163181	-	52	NuclAT_1	-	-
-	163130..163181	-	52	NuclAT_1	-	-
BSG22_RS30020	163667..163843	-	177	WP_001054900.1	hypothetical protein	-
BSG22_RS26145	164235..164444	+	210	WP_000062603.1	HEAT repeat domain-containing protein	-
BSG22_RS26150	164516..165178	-	663	WP_000644794.1	plasmid IncI1-type surface exclusion protein ExcA	-
BSG22_RS26155	165249..167417	-	2169	WP_071940810.1	DotA/TraY family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B / erm(42) / floR	-	1..207960	207960

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5817.21 Da
• Isoelectric Point: 8.7948

>T68720 WP_001303307.1 NZ_CP018104:162268-162420 [Escherichia coli]
MPQRTFLMMLIVICVTILCFVWMVRDSLCVLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T68720 NZ_CP018104:162268-162420 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCATCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGTACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin

Nucleotide

• Length: 63 bp

>AT68720 NZ_CP018104:c162218-162156 [Escherichia coli]
TCGAAGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: A0A634Q5L1

Antitoxin