Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2235795..2236297 Replicon chromosome
Accession NZ_CP018103
Organism Escherichia coli strain MRSN352231

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag BSG22_RS11765 Protein ID WP_000767829.1
Coordinates 2235795..2236049 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag BSG22_RS11770 Protein ID WP_001259255.1
Coordinates 2236046..2236297 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BSG22_RS11740 2230810..2231037 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
BSG22_RS11745 2231051..2232109 - 1059 WP_000492333.1 transport protein YeeE -
BSG22_RS11750 2232288..2233646 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
BSG22_RS29280 2233636..2233698 - 63 WP_010723108.1 membrane protein YoeI -
BSG22_RS11755 2233913..2234842 - 930 WP_000803351.1 LysR family transcriptional regulator -
BSG22_RS11760 2234888..2235712 - 825 WP_000754737.1 SDR family oxidoreductase -
BSG22_RS11765 2235795..2236049 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
BSG22_RS11770 2236046..2236297 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
BSG22_RS11775 2236580..2236630 + 51 WP_001364200.1 his operon leader peptide -
BSG22_RS11785 2236776..2237675 + 900 WP_000131792.1 ATP phosphoribosyltransferase -
BSG22_RS11790 2237681..2238985 + 1305 WP_001367667.1 histidinol dehydrogenase -
BSG22_RS11795 2238982..2240052 + 1071 WP_000108941.1 histidinol-phosphate transaminase -
BSG22_RS11800 2240052..2241119 + 1068 WP_000080141.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T68709 WP_000767829.1 NZ_CP018103:c2236049-2235795 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T68709 NZ_CP018103:c2236049-2235795 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT68709 WP_001259255.1 NZ_CP018103:c2236297-2236046 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT68709 NZ_CP018103:c2236297-2236046 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References