Detailed information of TA system TA068694

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3801257..3801850
• Replicon: chromosome
• Accession: NZ_CP018101
• Organism: Delftia sp. HK171

Toxin (Protein)

• Gene name: higB
• Uniprot ID: F6ASE3
• Locus tag: BO996_RS17115
• Protein ID: WP_013802475.1
• Coordinates: 3801257..3801535 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: BO996_RS17120
• Protein ID: WP_013802474.1
• Coordinates: 3801548..3801850 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BO996_RS17090	3796524..3797231	-	708	WP_047218695.1	fumarylacetoacetate hydrolase family protein	-
BO996_RS17095	3797270..3798307	-	1038	WP_071955826.1	cupin domain-containing protein	-
BO996_RS17100	3798324..3799532	-	1209	WP_071955828.1	3-hydroxybenzoate 6-monooxygenase	-
BO996_RS17105	3799737..3800273	+	537	WP_071955830.1	MarR family winged helix-turn-helix transcriptional regulator	-
BO996_RS17110	3800288..3801190	+	903	WP_063326189.1	LysR family transcriptional regulator	-
BO996_RS17115	3801257..3801535	+	279	WP_013802475.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BO996_RS17120	3801548..3801850	+	303	WP_013802474.1	HigA family addiction module antidote protein	Antitoxin
BO996_RS17125	3801966..3803006	+	1041	WP_071955832.1	patatin-like phospholipase family protein	-
BO996_RS17130	3803175..3803630	+	456	WP_071955834.1	DUF2846 domain-containing protein	-
BO996_RS17135	3803718..3804992	-	1275	WP_071955836.1	serine hydroxymethyltransferase	-
BO996_RS17140	3805039..3806277	-	1239	WP_083571171.1	aspartate aminotransferase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	3737905..3805099	67194

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (6-83)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10518.17 Da
• Isoelectric Point: 9.2618

>T68694 WP_013802475.1 NZ_CP018101:3801257-3801535 [Delftia sp. HK171]
MILDFRCADTQALMLGKRVARFANLEAAAMRKLVQLNAATSLEFLRIPPGNRLEALQGSRRGQYSIRINEQWRLCFVWAD
GHASQVEIVDYH

Nucleotide

• Length: 279 bp

>T68694 NZ_CP018101:3801257-3801535 [Delftia sp. HK171]
ATGATCCTCGATTTCAGATGTGCAGACACCCAGGCATTGATGCTTGGCAAGCGCGTTGCGCGGTTCGCCAACCTGGAGGC
CGCTGCCATGCGCAAGCTTGTGCAGCTGAACGCCGCGACCTCCCTGGAGTTCCTGCGCATTCCGCCAGGCAACAGACTGG
AGGCCCTTCAAGGAAGCCGCAGGGGCCAGTACAGCATTCGGATCAATGAGCAATGGCGCCTTTGCTTCGTCTGGGCAGAT
GGCCATGCCAGCCAGGTCGAGATCGTGGATTACCACTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11364.06 Da
• Isoelectric Point: 5.1007

>AT68694 WP_013802474.1 NZ_CP018101:3801548-3801850 [Delftia sp. HK171]
MTREVKWPHPGEILMQEWLEPMGLSQYALAKAIDVPPRRINEIVKGMRGITADTALRLAVFFGTDAQSWLNLQSDYDLAE
ARDAMADVLARIQRFEPSHP

Nucleotide

• Length: 303 bp

>AT68694 NZ_CP018101:3801548-3801850 [Delftia sp. HK171]
ATGACCCGCGAAGTCAAATGGCCGCACCCAGGAGAAATCCTGATGCAGGAGTGGCTCGAACCCATGGGCCTGAGCCAGTA
CGCGTTGGCAAAGGCCATCGATGTGCCGCCCCGGCGCATCAACGAGATCGTGAAGGGCATGCGCGGCATCACGGCGGATA
CGGCGCTGCGCCTTGCGGTCTTCTTTGGCACGGACGCGCAAAGCTGGCTCAACCTGCAGTCCGACTACGACCTTGCCGAA
GCCCGCGATGCCATGGCGGACGTGCTGGCGCGAATCCAGCGGTTTGAGCCCTCGCATCCCTGA

Structures

Toxin

• AlphaFold DB: A0A031I1T8