Detailed information of TA system TA068691

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1375966..1376640
• Replicon: chromosome
• Accession: NZ_CP018101
• Organism: Delftia sp. HK171

Toxin (Protein)

• Gene name: graT
• Uniprot ID: -
• Locus tag: BO996_RS06245
• Protein ID: WP_043789322.1
• Coordinates: 1376359..1376640 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: BO996_RS06240
• Protein ID: WP_012203141.1
• Coordinates: 1375966..1376277 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BO996_RS06230	1371455..1372477	+	1023	WP_071954289.1	type I-F CRISPR-associated endonuclease Cas1	-
BO996_RS06235	1372474..1375962	+	3489	WP_071954290.1	type I-F CRISPR-associated helicase Cas3	-
BO996_RS06240	1375966..1376277	-	312	WP_012203141.1	HigA family addiction module antidote protein	Antitoxin
BO996_RS06245	1376359..1376640	-	282	WP_043789322.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BO996_RS06250	1376807..1378156	+	1350	WP_071954291.1	type I-F CRISPR-associated protein Csy1	-
BO996_RS06255	1378153..1379235	+	1083	WP_071954292.1	type I-F CRISPR-associated protein Csy2	-
BO996_RS06260	1379290..1380318	+	1029	WP_071954293.1	type I-F CRISPR-associated protein Csy3	-
BO996_RS06265	1380324..1380890	+	567	WP_065345397.1	type I-F CRISPR-associated endoribonuclease Cas6/Csy4	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-93)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-98)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10721.30 Da
• Isoelectric Point: 8.5442

>T68691 WP_043789322.1 NZ_CP018101:c1376640-1376359 [Delftia sp. HK171]
MAITSFKCPETQRLFTSGKTRRFANIQSVAERKLAQLDAAVTLEFLRSPPGNRLEMLVGDRQGQHSIRINDQWRLCFVWT
EKGVVDVEIVDYH

Nucleotide

• Length: 282 bp

>T68691 NZ_CP018101:c1376640-1376359 [Delftia sp. HK171]
ATGGCGATCACTTCCTTCAAGTGCCCGGAAACCCAGCGCCTGTTCACCAGCGGCAAGACCCGCCGGTTCGCCAACATCCA
GAGCGTGGCGGAGCGCAAGCTGGCTCAGTTGGATGCGGCTGTGACACTTGAATTTCTGCGCAGCCCTCCTGGCAATCGCC
TGGAAATGCTCGTGGGAGATCGGCAGGGCCAGCACAGCATCCGCATCAACGACCAGTGGCGCCTCTGCTTTGTCTGGACG
GAAAAGGGAGTGGTTGACGTAGAGATAGTGGATTACCACTGA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11615.32 Da
• Isoelectric Point: 8.6927

>AT68691 WP_012203141.1 NZ_CP018101:c1376277-1375966 [Delftia sp. HK171]
MLKNGMRPIHPGEVLREDYLKPMDMSANALARHLHVPASRVNDIVLERRGITADTALRLSRFFGGDAQSWLNLQTAYDLR
TAELNAELQASVRQVHPMQLHTA

Nucleotide

• Length: 312 bp

>AT68691 NZ_CP018101:c1376277-1375966 [Delftia sp. HK171]
ATGCTTAAAAACGGCATGCGCCCCATACACCCAGGTGAAGTCCTGCGCGAGGACTACCTGAAGCCCATGGACATGAGCGC
CAACGCGCTGGCCAGGCACCTGCACGTTCCCGCCTCGCGCGTGAACGACATCGTGCTGGAGCGGCGCGGCATCACGGCCG
ACACGGCGCTGCGGCTGAGCCGCTTCTTTGGGGGCGACGCGCAGTCCTGGCTGAACCTTCAGACCGCCTACGACCTGCGC
ACGGCAGAGCTGAACGCGGAGTTGCAGGCGTCCGTGAGGCAAGTTCATCCGATGCAGCTGCATACCGCCTGA